运动和2,3,7,8-四氯二苯并二噁英(TCDD)持续暴露对大鼠肾脏氧化应激指标的影响
Effects of Exercise and Continuous Exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on Renal Oxidative Stress Indexes in Rats
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摘要: 观察8周有氧运动和2,3,7,8-四氯二苯并二噁英(2,3,7,8-tetrachlorodibenzo-p-dioxin, 2,3,7,8-TCDD)持续暴露对大鼠肾脏组织氧化应激指标及相关蛋白水平的影响。选取40只8周龄雄性Sprague-Dawley(SD)大鼠，随机分为空白对照组(NC组)、运动组(EC组)、TCDD暴露组(NT组)和运动+TCDD暴露组(ET组)，NT组和ET组进行8周TCDD处理(腹腔注射，每周1次)首次剂量为6.4 μg·kg-1，第2～8周采用维持剂量(为首次剂量的21%)，EC组和ET组采用游泳运动干预方式(5%体质量的尾部负重，每周5次)。8周干预后，测定血清尿素氮(urea nitrogen, BUN)、肌酐(creatinine, Cr)，肾脏氧化应激相关指标脂质过氧化水平、谷胱甘肽(glutathione, GSH)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)、超氧化物歧化酶(superoxide dismutase, SOD)、过氧化氢酶(catalase, CAT)以及细胞色素P4501A1(cytochrome P4501A1, CYP1A1)蛋白含量。结果显示，运动可降低大鼠体质量和肾脏质量，提高GSH-Px活性；TCDD暴露可降低血清BUN水平，提高脂质过氧化水平、GSH含量、GSH-Px和SOD活性，提高CYP1A1蛋白含量；TCDD暴露下运动可提高GSH-Px活性，降低CYP1A1蛋白含量。本研究表明，TCDD暴露可导致大鼠肾脏发生氧化应激，可能与TCDD诱导的CYP1A1增加有关；8周中等强度有氧运动未能明显缓解TCDD诱导的氧化应激，但能显著降低TCDD诱导的CYP1A1水平，具体机制有待进一步研究。
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关键词:
- 2,3,7,8-四氯二苯并二噁英 /
- 大鼠 /
- 氧化应激 /
- 运动
Abstract: This study aims to investigate the renal oxidative stress indexes and related protein levels in response to exercise and 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) exposure. Forty 8-week-old male Sprague-Dawley (SD) rats were randomly divided into blank control group (NC group), exercise group (EC group), TCDD exposure group (NT group) and exercise+TCDD exposure group (ET group). Both NT and ET groups were treated with TCDD for 8 weeks (intraperitoneal injection, weekly). The initial dose was 6.4 μg·kg-1, followed by a maintenance dose (21% of the initial dose) from week 2 to 8. Exercise intervention were prescribed for both EC and ET groups in the form of swimming (tail loading at 5% of body weight, five times per week). After 8 weeks of intervention, serum urea nitrogen (BUN) and creatinine (Cr), indicators related to renal oxidative stress including lipid peroxidation level, glutathione (GSH), glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), catalase (CAT) and cytochrome P4501A1 (CYP1A1) protein relative content were measured. The results showed that exercise could reduce body weight and kidney weight, and increase GSH-Px activity. TCDD exposure resulted serum BUN reduction, and increased lipid peroxidation level, GSH content, GSH-Px and SOD activity, and CYP1A1 level. The combination of exercise intervention and TCDD exposure can increase GSH-Px activity and decrease CYP1A1 level. This study indicated that TCDD exposure can cause oxidative stress in rat kidneys, which may be related to the TCDD-induced increase of CYP1A1. Moderate-intensity aerobic exercise for 8 weeks could not significantly relieve TCDD-induced oxidative stress, but could significantly reduce the level of TCDD-induced CYP1A1. The specific mechanism needs further research.-
Key words:
- 2,3,7,8-TCDD /
- rat /
- oxidative stress /
- exercise
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