氟啶虫酰胺慢性暴露对斑马鱼神经行为影响研究
Neurobehavioral Effect of Chronic Flonicamid Exposure in Adult Zebrafish
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摘要: 氟啶虫酰胺是一种高选择性杀虫剂，对蚜虫等刺吸式口器害虫具有很好的神经毒性和快速拒食活性，是目前唯一被报道的作用于内向整流钾离子通道的杀虫剂。为了更科学、合理地使用氟啶虫酰胺，开展氟啶虫酰胺对环境非靶标生物毒性及其机制研究具有重要意义。本研究以斑马鱼（Danio rerio）为研究对象，研究环境相关浓度氟啶虫酰胺慢性暴露21 d对斑马鱼的神经行为毒性。结果表明，氟啶虫酰胺浓度为1、10和100 μg·L-1时，氟啶虫酰胺慢性暴露对被试斑马鱼成鱼焦虑样行为以及学习、记忆行为造成一定影响；被试斑马鱼多巴胺和皮质醇的含量有一定的增高趋势，但与对照组相比无显著差异（P>0.05）；与神经行为相关基因线粒体外膜蛋白2（mitoguardin 2，miga2）和黄嘌呤脱氢酶（xanthine dehydrogenase，xdh）的表达水平发生显著改变（P<0.05）；而腺苷脱氨酶（adenosine deaminase，ada）、干扰素调节因子1（interferon regulatory factor 1，irf1）和线粒体融合蛋白2（mitofusin 2，mfn2）3个基因的表达量无显著性影响（P>0.05）。因此，斑马鱼成鱼暴露于1、10和100 μg·L-1的氟啶虫酰胺21 d后，其行为出现异常，线粒体和免疫功能受到影响。本研究对氟啶虫酰胺对水生生物毒性风险预警具有重要的参考价值。Abstract: Flonicamid, a selective insecticide, is the only insecticide which is reported to act on the inwardly-rectifying potassium channels (Kir) at present. It exerts toxic effects against sap-sucking insects by inhibiting insect feeding and neurotoxicity. In order to use it rationally and scientifically to control pests, it is significant to study the toxicity of flonicamid to environmental non-target organisms and its mechanism. In this work, zebrafish (Danio rerio) is used to study the neurobehavioral toxicity of flonicamid by chronic exposure for 21 d with environmental related concentrations. We found that flonicamid showed a potential effect on anxiety-like behavior and learning-memory capacity of adult zebrafish at the concentrations of 10 μg·L-1 and 100 μg·L-1, and increased slightly the contents of dopamine and cortisol in the treated zebrafish. However, there was no significant difference compared to the control group (P>0.05). RT-qPCR revealed that the expression levels of miga2 (mitoguardin 2) and xdh (xanthine dehydrogenase) related to neurobehavior were changed significantly (P<0.05). The expression of ada (adenosine deaminase), irf1 (interferon regulatory factor 1) and mfn2 (mitofusin 2) genes showed no significant difference comparing with that in the control group (P>0.05). Therefore, flonicamid showed potential effects on the behavior, mitochondrial and immune function of zebrafish after exposed chronically to 1, 10 and 100 μg·L-1 for 21 d. This study provides a starting point to unveil the early warning of aquatic toxicity of flonicamid.
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Key words:
- flonicamid /
- zebrafish /
- neurobehavioral toxicity /
- anxiety-like behavior /
- learning-memory behavior
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