双酚P对斑马鱼的生长和发育毒性
Developmental Toxicity of Bisphenol P on Zebrafish
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摘要: 双酚A （bisphenol A，BPA）是一种典型的内分泌干扰物，目前许多国家出台了管控措施，因此一些BPA类似物，包括双酚P （bisphenol P，BPP）被开发用来替代BPA。目前很多研究发现BPA类似物具有与BPA相似的毒性效应，但是关于BPP毒性效应的研究还比较缺乏。本文以斑马鱼为研究模型，将胚胎暴露于0.1、0.2、0.3、0.4、0.6和0.8 mg·L-1的BPP中至受精后60 dpf （days post fertilization，dpf），研究BPP对斑马鱼的发育毒性和内分泌干扰效应。结果表明，斑马鱼7 dpf-孵化率的降低和8 dpf-累积死亡率的升高均具有剂量-效应关系，半数效应浓度（50% effective concentration，EC50）值分别为0.580 mg·L-1和0.247 mg·L-1。幼体生长阶段，30 dpf-畸形率的EC50值为0.380 mg·L-1；对于体长和体质量抑制的30 d-EC50≥0.6 mg·L-1。斑马鱼的“假定”特定生长率（specific growth rate，SGR）和浓度存在显著的剂量-效应关系，随着BPP浓度增高，假定特定生长率均呈下降趋势，并且45～60 d的体质量和体长特定生长率的抑制效应浓度比30～45 d更低。激素水平测定结果表明，BPP暴露显著降低了斑马鱼体内的雄激素水平，提高了雌激素和卵黄蛋白原的水平。本研究结果表明，BPP对斑马鱼胚胎及幼鱼的生长发育具有一定的毒性效应。Abstract: Bisphenol A (BPA) has been banned in certain products due to its endocrine disrupting effects. Giving the adverse effects, some bisphenol analogs such as bisphenol P (BPP) has been developed to substitute BPA in manufacture of polycarbonate and epoxy resins. Previous studies showed that bisphenol analogs possess similar effects in physiological systems as BPA. However, studies on toxic effects of BPP to aquatic organisms are still limited. In this study, zebrafish embryos were exposed to BPP until 60 days post fertilization (dpf) to investigate the developmental toxicity and endocrine disrupting effects of BPP. The results revealed that BPP inhibited the hatching rates of 7 dpf zebrafish larvae and increased the mortality of 8 dpf larvae in a dose-dependent manner, with 50% effective concentration (EC50) of about 0.580 mg·L-1 and 0.247 mg·L-1, respectively. For juvenile zebrafish, the EC50 value of inhibition of body length and weight of BPP was ≥ 0.6 mg·L-1. The specific growth rate (SGR) of zebrafish decreased in a dose-dependent manner after exposure to BPP. The effective BPP concentration on inhibition of body length and weight between 45~60 d was much lower than that between 30~45 d. In addition, homogenate testosterone (T) levels decreased, while 17β-estradiol (E2) and vitellogenin levels increased significantly in male zebrafish after exposure to BPP. The results demonstrated that the BPP exposure exhibited developmental toxicity on zebrafish.
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Key words:
- bisphenol analogs /
- bisphenol P /
- zebrafish /
- developmental toxicity /
- endocrine disrupting effects
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