硝基化合物的高效液相色谱(HPLC)分析

刘秀华; 朱方华; 何小波; 孟丹; 胥全敏

环境化学

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刘秀华, 朱方华, 何小波, 孟丹, 胥全敏. 硝基化合物的高效液相色谱(HPLC)分析[J]. 环境化学, 2012, 31(3): 385-389.

引用本文:

刘秀华, 朱方华, 何小波, 孟丹, 胥全敏. 硝基化合物的高效液相色谱(HPLC)分析[J]. 环境化学, 2012, 31(3): 385-389.

LIU Xiuhua, ZHU Fanghua, HE Xiaobo, MENG Dan, XU Quanmin. Studies on the HPLC analysis conditions of nitryl compounds[J]. Environmental Chemistry, 2012, 31(3): 385-389.

Citation:

LIU Xiuhua, ZHU Fanghua, HE Xiaobo, MENG Dan, XU Quanmin. Studies on the HPLC analysis conditions of nitryl compounds[J]. Environmental Chemistry, 2012, 31(3): 385-389.

硝基化合物的高效液相色谱(HPLC)分析

1.
中国工程物理研究院, 绵阳, 621900
基金项目:
中国工程物理研究环保基金(617)资助.

Studies on the HPLC analysis conditions of nitryl compounds

1.
China Academy of Engineering Physics, Mianyang, 621900, China
Fund Project:

摘要: 硝基化合物是炸药废水的主要成分,大部分硝基化合物具有较高的毒性.本文对奥克托今、黑索今、1,3,5-三硝基苯、1,3-二硝基苯、硝基苯、2,4,6-三硝基甲苯、2-氨基-4,6-二硝基甲苯、2,4-二硝基甲苯共8种硝基化合物的紫外光谱和液相色谱分离条件进行了研究,其最佳检测波长分别为228 nm、227 nm、227 nm、237 nm、272 nm、230 nm、226 nm、244 nm.本文建立了8种硝基化合物的高效液相色谱测定方法,色谱条件为:色谱柱为ZORBAX SB-C18(3.0 mm×250 mm, 5 μm),检测器为紫外检测器,流动相为甲醇-水(50:50),流速为0.5 mL·min-1.水中8种硝基化合物可以在13 min内得到较好的分离,检出限均≤0.8 ng,回收率大于95%.
- 硝基化合物 /
- 紫外光谱 /
- 液相色谱分析 /
- 炸药
Abstract: Nitro compounds are the main components of explosive wastewater. Most nitro compounds are poisonous. This paper studied the ultraviolet spectra and HPLC separation conditions of eight nitro compounds, including HMX, RDX, 1,3,5-trinitrobenze, 1,3-dinitrobenzeze, Nitrobenzene, TNT, 2-Amino-4,6-dinitrotoluene and 2,4-dinitrotoluene. Their optimal determination wavelengths are 228 nm, 227 nm, 227 nm, 237 nm, 272 nm, 230 nm, 226 nm, 244 nm respectively. The HPLC method is employed with ZORBAX SB-C18 column (3.0 mm×250 mm, 5 μm) and a UV detector. The mobile phase was methanol mingled with water (Vmethanol:Vwater=50:50) at a flow rate of 0.5 mL·min-1. The eight nitro compounds can be separated well in 13 min, the detection limits are less than 0.8 ng and the recoveries are more than 95%.
- nitro compounds /
- ultraviolet spectrum /
- HPLC Analysis /
- explosives

[1]	Sublette K L, Ganapatby E V, Schwartz S. Degradation of munition wastes by phanerochaere chrysosporium[J]. Appl Biochem Biotech, 1992, 34/35:709-723
[2]	Jeffery A, Steevens B, Maurice Duke, et al. Toxicity of the explosives 2,4,6-trinitrotoluene, hexahydro-1,3,5-trinitro-1,3,5-triazine, and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine in sediments to Chironomus tentans and Hyalella azteca: Low-dose hormesis and high-dose mortality[J]. Environmental Toxicology and Chemistry, 2002, 21(7):1475-1482
[3]	Won W D, Disalvo L H, Ng J, Toxicity and mutagenicity of 2,4,6-trinitrotoluene and its microbial metabolites[J]. Appl Environ Microbiol, 1976, 31(4):576-580
[4]	国家环境保护总局《水和废水监测分析方法》编委会. 水和废水监测分析方法(第四版增补版)[J]. 北京: 中国环境科学出版社, 2010
[5]	Birgitta Persson, Henric Östmark, Helena Bergman. An HPLC method for analysis of HNIW and TNAZ in an explosive mixture[J]. Propellants, Explosives, Pyrotechnics, 1997, 22(4):238-239
[6]	Tobias Bausinger, Johannes Preuss. Stability of nitroaromatic specialty explosives in reversed-phase liquid chromatographic systems[J]. Journal of Hazardous Materials,2009, 162(2/3):1578-1582
[7]	梁奕昌,义志忠,蔡志虹,等. 硝基甲苯与硝基苯甲酸异构体的高效液相色谱法分析[J].色谱,1999,17(4): 397-398
[8]	刘永刚,陈曙东,罗顺火.多组分炸药混合物中CL-20的高效液相色谱测定[J].化学研究与应用,2000,12(4): 446-448
[9]	宁艳利,王亚鑫,葛彦平,等.改性双基推进剂中HMX含量的高效液相色谱测定[J].含能材料,2005,13(4):252-254
[10]	张艳林,高翠莲,杨业全,等.固相萃取-高效液相色谱法检测水中硝基苯[J].贵州农业科学, 2009,37(7):238-239
[11]	宁永成.有机化合物结构鉴定与有机波谱学(第二版)[M]. 北京: 科学出版社, 2000
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刘秀华, 朱方华, 何小波, 孟丹, 胥全敏. 硝基化合物的高效液相色谱(HPLC)分析[J]. 环境化学, 2012, 31(3): 385-389.

引用本文:

刘秀华, 朱方华, 何小波, 孟丹, 胥全敏. 硝基化合物的高效液相色谱(HPLC)分析[J]. 环境化学, 2012, 31(3): 385-389.

LIU Xiuhua, ZHU Fanghua, HE Xiaobo, MENG Dan, XU Quanmin. Studies on the HPLC analysis conditions of nitryl compounds[J]. Environmental Chemistry, 2012, 31(3): 385-389.

Citation:

LIU Xiuhua, ZHU Fanghua, HE Xiaobo, MENG Dan, XU Quanmin. Studies on the HPLC analysis conditions of nitryl compounds[J]. Environmental Chemistry, 2012, 31(3): 385-389.

硝基化合物的高效液相色谱(HPLC)分析

1. 中国工程物理研究院, 绵阳, 621900

收稿日期: 2011-03-28

基金项目:

中国工程物理研究环保基金(617)资助.

关键词:

摘要: 硝基化合物是炸药废水的主要成分,大部分硝基化合物具有较高的毒性.本文对奥克托今、黑索今、1,3,5-三硝基苯、1,3-二硝基苯、硝基苯、2,4,6-三硝基甲苯、2-氨基-4,6-二硝基甲苯、2,4-二硝基甲苯共8种硝基化合物的紫外光谱和液相色谱分离条件进行了研究,其最佳检测波长分别为228 nm、227 nm、227 nm、237 nm、272 nm、230 nm、226 nm、244 nm.本文建立了8种硝基化合物的高效液相色谱测定方法,色谱条件为:色谱柱为ZORBAX SB-C18(3.0 mm×250 mm, 5 μm),检测器为紫外检测器,流动相为甲醇-水(50:50),流速为0.5 mL·min-1.水中8种硝基化合物可以在13 min内得到较好的分离,检出限均≤0.8 ng,回收率大于95%.

English Abstract

Studies on the HPLC analysis conditions of nitryl compounds

1. China Academy of Engineering Physics, Mianyang, 621900, China

Received Date: 2011-03-28

Fund Project:

Keywords:

Abstract: Nitro compounds are the main components of explosive wastewater. Most nitro compounds are poisonous. This paper studied the ultraviolet spectra and HPLC separation conditions of eight nitro compounds, including HMX, RDX, 1,3,5-trinitrobenze, 1,3-dinitrobenzeze, Nitrobenzene, TNT, 2-Amino-4,6-dinitrotoluene and 2,4-dinitrotoluene. Their optimal determination wavelengths are 228 nm, 227 nm, 227 nm, 237 nm, 272 nm, 230 nm, 226 nm, 244 nm respectively. The HPLC method is employed with ZORBAX SB-C18 column (3.0 mm×250 mm, 5 μm) and a UV detector. The mobile phase was methanol mingled with water (Vmethanol:Vwater=50:50) at a flow rate of 0.5 mL·min-1. The eight nitro compounds can be separated well in 13 min, the detection limits are less than 0.8 ng and the recoveries are more than 95%.

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硝基化合物的高效液相色谱(HPLC)分析

Studies on the HPLC analysis conditions of nitryl compounds

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硝基化合物的高效液相色谱(HPLC)分析

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Studies on the HPLC analysis conditions of nitryl compounds

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