骨炭、过磷酸钙对稀土矿区停耕稻田土壤细菌群落的影响
Effects of Bone Char and Superphosphate on Bacterial Community in Soil of Fallow Paddy Fields in Rare Earth Mining Area
-
摘要: 磷是农作物生长和高产必不可少的元素，细菌是揭示土壤生态环境演变的重要指示剂。运用盆栽实验探讨骨炭、过磷酸钙对稀土矿区土壤细菌群落的影响。研究表明，含磷材料对稀土矿区停耕土壤细菌群落的影响与P含量、pH、稀土元素离子浓度，时间、温度等因素相关。过磷酸钙添加40 d后、骨炭添加80 d后，稀土矿区停耕稻田土壤细菌群落Chao、Ace指数均有升高，土壤细菌群落多样性增大；pH是影响细菌群落结构和功能的关键因子，酸杆菌门(Acidobacteria)与pH显著负相关，疣微菌门(Verrucomicrobia)与pH显著正相关；骨炭、过磷酸钙中的P促进变形菌门(Proteobacteria)的生长，无论是40 d，还是80 d时，骨炭处理与过磷酸钙处理的土壤变形菌门(Proteobacteria)所占比例均大于对照处理；骨炭、过磷酸钙中的磷酸根离子与土壤中的稀土元素反应形成磷酸稀土沉淀，减少了溶液中的稀土元素浓度，导致骨炭、过磷酸钙处理的土壤柯氏假丝酵母菌(Candidatus_koribacter)丰度低于对照处理；土壤细菌群落结构受时间、温度等因素的影响，骨炭、过磷酸钙、对照处理40～80 d时，随着时间、温度的变化，疣微菌门(Verrucomicrobia)丰度发生了显著变化。研究表明，骨炭、过磷酸钙对稀土矿区停耕稻田均有一定的修复效果。Abstract: Phosphorus (P) is an essential element for growth and high yield of crops, while bacteria are important indicators to reveal the evolution of soil ecological environment. The effects of bone char (BC) and superphosphate (SSP) on soil bacterial community in rare earth mining area were studied by pot experiments. The research showed that the effects of phosphorus materials on soil bacterial community were related to P content, pH, ion concentration of rare earth elements, time and temperature and other factors. After adding SSP for 40 d and BC for 80 d, the indexes of Chao and Ace of soil bacterial community both increased, and the diversity of soil bacterial community also increased. pH was the key factor affecting the structure and function of bacterial community. Acidobacteria was negatively correlated with pH while Verrucomicrobia was positively correlated with pH. Phosphorus in BC and SSP promotes the growth of Proteobacteria and the proportion of Proteobacteria in the soil treated with BC and SSP was higher than that of the control check (CK) samples whether after 40 d or 80 d. The proportion of Proteobacteria in soil treated with BC and SSP was higher than that of CK, no matter 40 d or 80 d later. P ions in BC and SSP react with rare earth elements in soil to form rare earth phosphate precipitation and reduce the concentration of rare earth elements in solution, which results in that the abundance of Candidatus_koribacter in soil treated with BC and SSP was lower than that of CK samples. Soil bacterial community structure was affected by time and temperature. The abundance of Verrucomicrobia of soil changed significantly with the change of time and temperature when treated with BC, SSP and CK for 40~80 d. The results also showed that BC and SSP had a certain restoration effect on the fallow paddy fields in the rare earth mining area.
-
Key words:
- rare earth elements /
- bacterial community /
- bone charcoal /
- superphosphate /
- soil /
- rare earth mining area
-
-
Jin S L, Jin W, Dong C X, et al. Effects of rice straw and rice straw ash on rice growth and α -diversity of bacterial community in rare-earth mining soils[J]. Scientific Reports, 2020, 10(1):10331 Jin W, Hu Z J, Bai Y J, et al. Response of rice and bacterial communities to the incorporation of rice straw in areas mined for heavy rare earth elements[J]. Bioresources, 2019, 14:9392-9409 金姝兰,黄益宗,胡莹,等.江西典型稀土矿区土壤和农作物中稀土元素含量及其健康风险评价[J].环境科学学报, 2014, 34(12):3084-3093 Jin S L, Huang Y Z, Hu Y, et al. Rare earth elements content and health risk assessment of soil and crops in typical rare earth mine area in Jiangxi Province[J]. Acta Scientiae Circumstantiae, 2014, 34(12):3084-3093(in Chinese)
高志强,周启星.稀土矿露天开采过程的污染及对资源和生态环境的影响[J].生态学杂志, 2011, 30(12):2915-2922 Gao Z Q, Zhou Q X. Contamination from rare earth ore strip mining and its impacts on resources and eco-environment[J]. Chinese Journal of Ecology, 2011, 30(12):2915-2922(in Chinese)
金姝兰,黄益宗.土壤中稀土元素的生态毒性研究进展[J].生态毒理学报, 2014, 9(2):213-223 Jin S L, Huang Y Z. A review on ecological toxicity of rare earth elements in soil[J]. Asian Journal of Ecotoxicology, 2014, 9(2):213-223(in Chinese)
Jin S L, Hu Z J, Man B Y, et al. Application of phosphate-containing materials affects bioavailability of rare earth elements and bacterial community in soils[J]. Science China Technological Sciences, 2019, 62(9):1616-1627 Jin S L, Jin W, Bai Y J, et al. Response of rice and bacterial community to phosphorus-containing materials in soil-plant ecosystem of rare earth mining area[J]. Journal of Hazardous Materials, 2020, 381:121004 厉桂香,马克明.土壤微生物多样性海拔格局研究进展[J].生态学报, 2018, 38(5):1521-1529 Li G X, Ma K M. Progress in the study of elevational patterns of soil microbial diversity[J]. Acta Ecologica Sinica, 2018, 38(5):1521-1529(in Chinese)
马垒,郭志彬,王道中,等.长期三水平磷肥施用梯度对砂姜黑土细菌群落结构和酶活性的影响[J].土壤学报, 2019, 56(6):1459-1470 Ma L, Guo Z B, Wang D Z, et al. Effect of long-term application of phosphorus fertilizer on soil bacterial community structure and enzymatic activity in lime concretion black soil relative to P application rate[J]. Acta Pedologica Sinica, 2019, 56(6):1459-1470(in Chinese)
Zhong W H, Cai Z C. Long-term effects of inorganic fertilizers on microbial biomass and community functional diversity in a paddy soil derived from quaternary red clay[J]. Applied Soil Ecology, 2007, 36(2-3):84-91 Beauregard M S, Hamel C, Atul-Nayyar, et al. Long-term phosphorus fertilization impacts soil fungal and bacterial diversity but not AM fungal community in alfalfa[J]. Microbial Ecology, 2010, 59(2):379-389 Yi B, Zhang Q C, Gu C, et al. Effects of different fertilization regimes on nitrogen and phosphorus losses by surface runoff and bacterial community in a vegetable soil[J]. Journal of Soils and Sediments, 2018, 18(11):3186-3196 Teufel A G, Li W, Kiss A J, et al. Impact of nitrogen and phosphorus on phytoplankton production and bacterial community structure in two stratified Antarctic lakes:A bioassay approach[J]. Polar Biology, 2017, 40(5):1007-1022 Tan H, Barret M, Mooij M J, et al. Long-term phosphorus fertilisation increased the diversity of the total bacterial community and the phoD phosphorus mineraliser group in pasture soils[J]. Biology and Fertility of Soils, 2013, 49(6):661-672 Lagos L M, Acuña J J, Maruyama F, et al. Effect of phosphorus addition on total and alkaline phosphomonoesterase-harboring bacterial populations in ryegrass rhizosphere microsites[J]. Biology and Fertility of Soils, 2016, 52(7):1007-1019 Liu M, Liu J, Chen X F, et al. Shifts in bacterial and fungal diversity in a paddy soil faced with phosphorus surplus[J]. Biology and Fertility of Soils, 2018, 54(2):259-267 Hamel C, Hanson K, Selles F, et al. Seasonal and long-term resource-related variations in soil microbial communities in wheat-based rotations of the Canadian prairie[J]. Soil Biology and Biochemistry, 2006, 38(8):2104-2116 赵国强,王淑平,崔骁勇,等.青藏高原高寒草原土壤微生物量对氮磷肥添加的响应[J].中国科学院大学学报, 2018, 35(3):417-424 Zhao G Q, Wang S P, Cui X Y, et al. Effects of nitrogen and phosphorus application on soil microbial biomass carbon and nitrogen contents on an alpine grassland on Tibetan Plateau[J]. Journal of University of Chinese Academy of Sciences, 2018, 35(3):417-424(in Chinese)
Jin S L, Hu Z J, Huang Y Z, et al. Evaluation of several phosphate amendments on rare earth element concentrations in rice plant and soil solution by X-ray diffraction[J]. Chemosphere, 2019, 236:124322 安凤秋.外源重金属铅和镉对土壤生物活性及微生物群落多样性的影响研究[D].杨凌:西北农林科技大学, 2018:13-16An F Q. Influence on soil biological activity and bacteria community diversity by exogenous lead and cadmium[D]. Yangling:Northwest A&F University, 2018:13 -16(in Chinese)
Zhou J Z, Xia B C, Treves D S, et al. Spatial and resource factors influencing high microbial diversity in soil[J]. Applied and Environmental Microbiology, 2002, 68(1):326-334 姜照伟,翁伯琦,黄元仿,等.镧对土壤微生物的影响[J].中国稀土学报, 2008, 26(4):498-502 Jiang Z W, Weng B Q, Huang Y F, et al. Effects of lanthanum on soil microorganism[J]. Journal of the Chinese Rare Earth Society, 2008, 26(4):498-502(in Chinese)
王友生,侯晓龙,蔡丽平,等.稀土开采对土壤细菌群落组成与多样性的影响[J].中国环境科学, 2017, 37(8):3089-3095 Wang Y S, Hou X L, Cai L P, et al. Impacts of rare earth mining on soil bacterial community composition and biodiversity[J]. China Environmental Science, 2017, 37(8):3089-3095(in Chinese)
王建宏.包钢尾矿坝外源稀土积累区土壤微生物特征研究[D].呼和浩特:内蒙古师范大学, 2011:23, 41-42Wang J H. Study on characteristics of soil microbe in exogenous rare earths accumulation area of Baogang tailings dam[D]. Hohhot:Inner Mongolia Normal University, 2011 :23, 41-42(in Chinese)
郎明.长期施用磷肥土壤微生物的群落结构特征及适应性探究[D].北京:中国农业大学, 2018:18-19, 21Lang M. Community structure and adaptation of soil microbiome after long-term phosphorus fertilization[D]. Beijing:China Agricultural University, 2018 :18-19, 21(in Chinese)
金姝兰,胡忠俊,邱秋萍,等.含磷材料对土壤溶液稀土元素含量的影响[J].生态学杂志, 2018, 37(6):1693-1701 Jin S L, Hu Z J, Qiu Q P, et al. Effects of phosphorated materials on the concentrations of rare earth elements in soil solution[J]. Chinese Journal of Ecology, 2018, 37(6):1693-1701(in Chinese)
王民煌.地上/地下碳输入和养分添加对滨海沙地人工林土壤微生物群落结构和功能的影响[D].福州:福建师范大学, 2018:63Wang M H. The effect of above/belowground carbon inputs and nutrient inputs on soil microbial community structure and function in a coastal dune forest[D]. Fuzhou:Fujian Normal University, 2018:63(in Chinese) 季卫东. DRD4exonⅢ48bpVNTR等5个功能基因多态性和汉族抽动秽语综合症及其认知功能关系的研究[D].成都:四川大学, 2005:47-48, 71Ji W D. A study on the polymorphism of DRD4 exonⅢ 48bp VNTR, DAT1, COMTmet/val, IL-1Ra86bp, IL-1β exon5 gene and cognitive function in Han Chinese children with Tourette's syndrome[D]. Chengdu:Sichuan University, 2005:47-48, 71(in Chinese)
Bokulich N A, Subramanian S, Faith J J, et al. Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing[J]. Nature Methods, 2013, 10(1):57-59 Kunin V, Engelbrektson A, Ochman H, et al. Wrinkles in the rare biosphere:Pyrosequencing errors can lead to artificial inflation of diversity estimates[J]. Environmental Microbiology, 2010, 12(1):118-123 Zhou J, Guan D W, Zhou B K, et al. Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China[J]. Soil Biology and Biochemistry, 2015, 90:42-51 杨胜香,李凤梅,彭禧柱,等.不同碳氮磷源改良剂对铅锌尾矿废弃地土壤微生物群落结构的影响[J].农业环境科学学报, 2019, 38(6):1256-1264 Yang S X, Li F M, Peng X Z, et al. Effects of amendments with different C/N/P ratios on the microbial community structure in Pb-Zn mine tailings[J]. Journal of Agro-Environment Science, 2019, 38(6):1256-1264(in Chinese)
理鹏,吴建强,沙晨燕,等.粪肥和有机肥施用对稻田土壤微生物群落多样性影响[J].环境科学, 2020, 41(9):4262-4272 Li P, Wu J Q, Sha C Y, et al. Effects of manure and organic fertilizer application on soil microbial community diversity in paddy fields[J]. Environmental Science, 2020, 41(9):4262-4272(in Chinese)
Gonzalez-Martinez A, Rodriguez-Sanchez A, Garcia-Ruiz M J, et al. Performance and bacterial community dynamics of a CANON bioreactor acclimated from high to low operational temperatures[J]. Chemical Engineering Journal, 2016, 287:557-567 Ma Q, Qu Y Y, Shen W L, et al. Bacterial community compositions of coking wastewater treatment plants in steel industry revealed by Illumina high-throughput sequencing[J]. Bioresource Technology, 2015, 179:436-443 林海,崔轩,董颖博,等.铜尾矿库重金属Cu、Zn对细菌群落结构的影响[J].中国环境科学, 2014, 34(12):3182-3188 Lin H, Cui X, Dong Y B, et al. Impact on bacterial community structure of heavy mental of Cu and Zn in copper mine tailings[J]. China Environmental Science, 2014, 34(12):3182-3188(in Chinese)
林耀奔,叶艳妹,吴次芳,等.水田土壤细菌群落对不同重金属污染水平的响应分析——以A县为例[J].环境科学学报, 2020, 40(1):224-233 Lin Y B, Ye Y M, Wu C F, et al. Response analysis of soil bacterial community to different heavy metal pollution levels in paddy fields:A case study of A County[J]. Acta Scientiae Circumstantiae, 2020, 40(1):224-233(in Chinese)
王光华,刘俊杰,于镇华,等.土壤酸杆菌门细菌生态学研究进展[J].生物技术通报, 2016, 32(2):14-20 Wang G H, Liu J J, Yu Z H, et al. Research progress of acidobacteria ecology in soils[J]. Biotechnology Bulletin, 2016, 32(2):14-20(in Chinese)
Chen D, Wei L, Zou Z C, et al. Bacterial communities in a novel three-dimensional bioelectrochemical denitrification system:The effects of pH[J]. Applied Microbiology and Biotechnology, 2016, 100(15):6805-6813 刘彩霞,董玉红,焦如珍.森林土壤中酸杆菌门多样性研究进展[J].世界林业研究, 2016, 29(6):17-22 Liu C X, Dong Y H, Jiao R Z. Research progress in acidobacteria diversity in forest soil[J]. World Forestry Research, 2016, 29(6):17-22(in Chinese)
汪涵.酸性矿山废水长期灌溉稻田土壤中微生物群落结构时空变化特征及其响应机制[D].广州:华南理工大学, 2018:27, 33Wang H. Characteristics of microbial community spatial and temporal change in paddy soil under long-term acid mine drainage irrigation and its response mechanism[D]. Guangzhou:South China University of Technology, 2018:27 , 33(in Chinese)
Jin S L, Hu Z J, Huang Y Z, et al. Evaluation of several phosphate amendments on rare earth element concentrations in rice plant and soil solution by X-ray diffraction[J]. Chemosphere, 2019, 236:124322 Ding L J, Su J Q, Sun G X, et al. Increased microbial functional diversity under long-term organic and integrated fertilization in a paddy soil[J]. Applied Microbiology and Biotechnology, 2018, 102(4):1969-1982 -
点击查看大图
计量
- 文章访问数: 2757
- HTML全文浏览数: 2757
- PDF下载数: 106
- 施引文献: 0
下载:
