异菌脲在油菜植株、土壤和水中的代谢途径及代谢物3,5-DCA的毒性研究

汪震, 华修德, 施海燕, 王鸣华. 异菌脲在油菜植株、土壤和水中的代谢途径及代谢物3,5-DCA的毒性研究[J]. 生态毒理学报, 2022, 17(4): 378-385. doi: 10.7524/AJE.1673-5897.20210308003
引用本文: 汪震, 华修德, 施海燕, 王鸣华. 异菌脲在油菜植株、土壤和水中的代谢途径及代谢物3,5-DCA的毒性研究[J]. 生态毒理学报, 2022, 17(4): 378-385. doi: 10.7524/AJE.1673-5897.20210308003
Wang Zhen, Hua Xiude, Shi Haiyan, Wang Minghua. Studies on Metabolic Pathways of Iprodione in Rape Plant, Soil, and Water and Toxicity of Metabolite 3,5-DCA[J]. Asian Journal of Ecotoxicology, 2022, 17(4): 378-385. doi: 10.7524/AJE.1673-5897.20210308003
Citation: Wang Zhen, Hua Xiude, Shi Haiyan, Wang Minghua. Studies on Metabolic Pathways of Iprodione in Rape Plant, Soil, and Water and Toxicity of Metabolite 3,5-DCA[J]. Asian Journal of Ecotoxicology, 2022, 17(4): 378-385. doi: 10.7524/AJE.1673-5897.20210308003

异菌脲在油菜植株、土壤和水中的代谢途径及代谢物3,5-DCA的毒性研究

    作者简介: 汪震(1996—),男,博士研究生,研究方向为农药残留与环境毒理,E-mail:2020202059@stu.njau.edu.cn
    通讯作者: 王鸣华, E-mail: wangmha@njau.edu.cn
  • 基金项目:

    国家重点研发计划项目(2016YFD0200207)

  • 中图分类号: X171.5

Studies on Metabolic Pathways of Iprodione in Rape Plant, Soil, and Water and Toxicity of Metabolite 3,5-DCA

    Corresponding author: Wang Minghua, wangmha@njau.edu.cn
  • Fund Project:
  • 摘要: 为了明确异菌脲在作物和环境中的代谢及代谢产物3,5-二氯苯胺(3,5-DCA)的生态风险,通过水培试验、土壤孵育和水解试验,采用UPLC-TOF-MS/MS,鉴定了异菌脲在油菜植株、土壤和水中的代谢产物,推测了代谢途径,并研究了3,5-DCA对细胞和蚯蚓的毒性。在油菜植株中检测到4种异菌脲代谢产物(M1、M3、M6和M7),在土壤中发现了5种代谢产物(M1、M3、M6、M7和M8),在水中发现了7种代谢产物(M1、M2、M4、M5、M7、M8和M9)。根据代谢产物的化学结构,明确了异菌脲的代谢主要通过酰胺键的断裂及脱烷基化反应,并推测了异菌脲在油菜植株、土壤和水中的代谢途径。代谢产物3,5-DCA对Hep G2的细胞毒性(IC50为99.7 mg·L-1)是异菌脲(IC50为304.8 mg·L-1)的3.1倍,对赤子爱胜蚓的14 d急性毒性(LC50为31.56 mg·kg-1)是母体的15倍以上(LC50>500 mg·kg-1),表明异菌脲的代谢为增毒代谢。研究结果对异菌脲的环境安全性评价具有重要的意义。
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  • 收稿日期:  2021-03-08

异菌脲在油菜植株、土壤和水中的代谢途径及代谢物3,5-DCA的毒性研究

    通讯作者: 王鸣华, E-mail: wangmha@njau.edu.cn
    作者简介: 汪震(1996—),男,博士研究生,研究方向为农药残留与环境毒理,E-mail:2020202059@stu.njau.edu.cn
  • 南京农业大学植物保护学院, 南京 210095
基金项目:

国家重点研发计划项目(2016YFD0200207)

摘要: 为了明确异菌脲在作物和环境中的代谢及代谢产物3,5-二氯苯胺(3,5-DCA)的生态风险,通过水培试验、土壤孵育和水解试验,采用UPLC-TOF-MS/MS,鉴定了异菌脲在油菜植株、土壤和水中的代谢产物,推测了代谢途径,并研究了3,5-DCA对细胞和蚯蚓的毒性。在油菜植株中检测到4种异菌脲代谢产物(M1、M3、M6和M7),在土壤中发现了5种代谢产物(M1、M3、M6、M7和M8),在水中发现了7种代谢产物(M1、M2、M4、M5、M7、M8和M9)。根据代谢产物的化学结构,明确了异菌脲的代谢主要通过酰胺键的断裂及脱烷基化反应,并推测了异菌脲在油菜植株、土壤和水中的代谢途径。代谢产物3,5-DCA对Hep G2的细胞毒性(IC50为99.7 mg·L-1)是异菌脲(IC50为304.8 mg·L-1)的3.1倍,对赤子爱胜蚓的14 d急性毒性(LC50为31.56 mg·kg-1)是母体的15倍以上(LC50>500 mg·kg-1),表明异菌脲的代谢为增毒代谢。研究结果对异菌脲的环境安全性评价具有重要的意义。

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