不同实验条件下稀有鮈鲫胚胎的自然受精率和孵化率

王绿平, 张京佶, 殷浩文. 不同实验条件下稀有鮈鲫胚胎的自然受精率和孵化率[J]. 生态毒理学报, 2020, 15(3): 100-107. doi: 10.7524/AJE.1673-5897.20190507001
引用本文: 王绿平, 张京佶, 殷浩文. 不同实验条件下稀有鮈鲫胚胎的自然受精率和孵化率[J]. 生态毒理学报, 2020, 15(3): 100-107. doi: 10.7524/AJE.1673-5897.20190507001
Wang Lvping, Zhang Jingji, Yin Haowen. Natural Fertility and Hatchability of Gobiocypris rarus Embryos under Different Experimental Conditions[J]. Asian Journal of Ecotoxicology, 2020, 15(3): 100-107. doi: 10.7524/AJE.1673-5897.20190507001
Citation: Wang Lvping, Zhang Jingji, Yin Haowen. Natural Fertility and Hatchability of Gobiocypris rarus Embryos under Different Experimental Conditions[J]. Asian Journal of Ecotoxicology, 2020, 15(3): 100-107. doi: 10.7524/AJE.1673-5897.20190507001

不同实验条件下稀有鮈鲫胚胎的自然受精率和孵化率

    作者简介: 王绿平(1986-),女,硕士,工程师,研究方向为水生生态毒理学,E-mail:wanglp@apm.sh.cn
  • 基金项目:

    上海市自然科学基金资助项目(17ZR1424400)

  • 中图分类号: X171.5

Natural Fertility and Hatchability of Gobiocypris rarus Embryos under Different Experimental Conditions

  • Fund Project:
  • 摘要: 以中国本土鱼种稀有鮈鲫(Gobiocypris rarus)胚胎为实验生物,研究稀有鮈鲫胚胎在正常光照条件下(温度(26±1)℃,自然光照)和光照周期颠倒条件下(温度(26±1)℃,光照周期为12 h(暗):12 h(光))的96 h自然受精率和孵化率,并在放大倍数为40倍的体视显微镜下观察稀有鮈鲫在胚胎发育过程中的形态发育时序及特征。结果表明,在光照周期颠倒和(26±1)℃条件下,9次重复试验的稀有鮈鲫胚胎自然受精率和孵化率平均值分别为92.2%和92.8%,变异系数(CV)分别为5.65%和6.21%,比正常光照和(26±1)℃条件下稀有鮈鲫胚胎的自然受精率和孵化率更高,为鱼类胚胎试验提供更高质量的胚胎。稀有鮈鲫胚胎的形态发育时序及特征满足胚胎试验观察的需求,可在特定的时间点观察特定表征。
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  • 收稿日期:  2019-05-07

不同实验条件下稀有鮈鲫胚胎的自然受精率和孵化率

    作者简介: 王绿平(1986-),女,硕士,工程师,研究方向为水生生态毒理学,E-mail:wanglp@apm.sh.cn
  • 上海市检测中心生物与安全实验室, 上海 201203
基金项目:

上海市自然科学基金资助项目(17ZR1424400)

摘要: 以中国本土鱼种稀有鮈鲫(Gobiocypris rarus)胚胎为实验生物,研究稀有鮈鲫胚胎在正常光照条件下(温度(26±1)℃,自然光照)和光照周期颠倒条件下(温度(26±1)℃,光照周期为12 h(暗):12 h(光))的96 h自然受精率和孵化率,并在放大倍数为40倍的体视显微镜下观察稀有鮈鲫在胚胎发育过程中的形态发育时序及特征。结果表明,在光照周期颠倒和(26±1)℃条件下,9次重复试验的稀有鮈鲫胚胎自然受精率和孵化率平均值分别为92.2%和92.8%,变异系数(CV)分别为5.65%和6.21%,比正常光照和(26±1)℃条件下稀有鮈鲫胚胎的自然受精率和孵化率更高,为鱼类胚胎试验提供更高质量的胚胎。稀有鮈鲫胚胎的形态发育时序及特征满足胚胎试验观察的需求,可在特定的时间点观察特定表征。

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