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Dong Shanshan, Zhang Dini, Yu Cigang, Wang Changyong, Liu Yan. Ecotoxicological Effects of Transgenic Cry1Ie Maize and Transgenic CP4-EPSPS Soybean on Zebrafish[J]. Asian Journal of Ecotoxicology, 2021, 16(5): 271-284. doi: 10.7524/AJE.1673-5897.20201215003
Citation: Dong Shanshan, Zhang Dini, Yu Cigang, Wang Changyong, Liu Yan. Ecotoxicological Effects of Transgenic Cry1Ie Maize and Transgenic CP4-EPSPS Soybean on Zebrafish[J]. Asian Journal of Ecotoxicology, 2021, 16(5): 271-284. doi: 10.7524/AJE.1673-5897.20201215003
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Ecotoxicological Effects of Transgenic Cry1Ie Maize and Transgenic CP4-EPSPS Soybean on Zebrafish

  • Key Laboratory on Biosafety of Environmental Protection, Nanjing Institute of Environmental Science, Ministry of Ecology and Environment, Nanjing 210042, China

  • Corresponding author: Liu Yan, liuyan@nies.org
  • Received Date: 15/12/2020
    Fund Project:
  • To evaluate the ecotoxicological effects of transgenic Cry1Ie insect-resistant maize and transgenic CP4-EPSPS herbicide-tolerant soybean on fish, zebrafish (Danio rerio) was used as a model animal and four experimental diets, including genetically modified (GM) maize, GM soybean, and corresponding near-isogenic non-GM lines were prepared respectively. A commercial feed was used as a reference diet. The feed intake, growth, reproduction, and antioxidant enzyme activity were investigated during a 98-day feeding trial. The histopathology and mRNA expression levels of sensitive protein were analyzed at different feeding stages. The results revealed that insect-resistant GM maize and herbicide-resistant GM soybean had no significant effects on growth performance, histopathology of the liver, intestinal tract, and brain, fecundity, and hatching rate of fertilized eggs. The activity of superoxide dismutase (SOD) was significantly higher in the liver of zebrafish fed with GM soybean compared with in those fed with the non-GM soybean and commercial diets, and was higher in male than in female fish (P<0.05). There were no obvious regular changes over time of mRNA expression levels of sensitive protein in zebrafish organ tissues. There were significant interaction effects between feeding duration, diet, and sex. When comparing experimental diets and commercial feed, we detected significant differences in growth performance, SOD activity in the liver, and mRNA expression levels, which may be related to the differences in palatability and nutrient composition of diets. In general, transgenic Cry1Ie insect-resistant maize and transgenic CP4-EPSPS herbicide-tolerant soybean have no obvious ecotoxicological effects on zebrafish.
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  • 国际农业生物技术应用服务组织. 2019年全球生物技术/转基因作物商业化发展态势[J]. 中国生物工程杂志, 2021, 41(1):114-119 International Service for the Acquisition of Agri-Biotech Applications. The global status of commercialized biotech/GM crops in 2019[J]. China Biotechnology, 2021, 41(1):114-119(in Chinese)

    Google Scholar Pub Med

    Strain K E, Whiting S A, Lydy M J. Laboratory and field validation of a Cry1Ab protein quantitation method for water[J]. Talanta, 2014, 128:109-116

    Google Scholar Pub Med

    Whiting S A, Strain K E, Campbell L A, et al. A multi-year field study to evaluate the environmental fate and agronomic effects of insecticide mixtures[J]. Science of the Total Environment, 2014, 497-498:534-542

    Google Scholar Pub Med

    Pott A, Otto M, Schulz R. Impact of genetically modified organisms on aquatic environments:Review of available data for the risk assessment[J]. Science of the Total Environment, 2018, 635:687-698

    Google Scholar Pub Med

    Douville M, Gagné F, André C, et al. Occurrence of the transgenic corn cry1Ab gene in freshwater mussels (Elliptio complanata) near corn fields:Evidence of exposure by bacterial ingestion[J]. Ecotoxicology and Environmental Safety, 2009, 72(1):17-25

    Google Scholar Pub Med

    陈秀萍, 王加美, 朱昊俊, 等. 转Bt基因作物对水生生态系统的影响研究进展[J]. 应用与环境生物学报, 2013, 19(4):569-574 Chen X P, Wang J M, Zhu H J, et al. Progress in effects of transgenic Bt crops on the aquatic ecosystem[J]. Chinese Journal of Applied and Environmental Biology, 2013, 19(4):569-574(in Chinese)

    Google Scholar Pub Med

    Harris J H. The use of fish in ecological assessments[J]. Austral Ecology, 1995, 20(1):65-80

    Google Scholar Pub Med

    董姗姗, 章嫡妮, 张振华, 等. 转基因作物对鱼类的生态毒理效应研究综述[J]. 生态毒理学报, 2017, 12(5):26-34 Dong S S, Zhang D N, Zhang Z H, et al. Overview of ecotoxicological effects of genetically modified crops on fish[J]. Asian Journal of Ecotoxicology, 2017, 12(5):26-34(in Chinese)

    Google Scholar Pub Med

    Sissener N H, Sanden M, Krogdahl Å, et al. Genetically modified plants as fish feed ingredients[J]. Canadian Journal of Fisheries and Aquatic Sciences, 2011, 68(3):563-574

    Google Scholar Pub Med

    Hemre G I, Sagstad A, Bakke-Mckellep A M, et al. Nutritional, physiological, and histological responses in Atlantic salmon, Salmo salar L. fed diets with genetically modified maize[J]. Aquaculture Nutrition, 2007, 13(3):186-199

    Google Scholar Pub Med

    Gu J N, Bakke A M, Valen E C, et al. Bt-maize (MON810) and non-GM soybean meal in diets for Atlantic salmon (Salmo salar L.) juveniles-Impact on survival, growth performance, development, digestive function, and transcriptional expression of intestinal immune and stress responses[J]. PLoS One, 2014, 9(6):e99932

    Google Scholar Pub Med

    Schlegel A, Stainier D Y R. Lessons from "lower" organisms:What worms, flies, and zebrafish can teach us about human energy metabolism[J]. PLoS Genetics, 2007, 3(11):e199

    Google Scholar Pub Med

    Seth A, Stemple D L, Barroso I. The emerging use of zebrafish to model metabolic disease[J]. Disease Models & Mechanisms, 2013, 6(5):1080-1088

    Google Scholar Pub Med

    Gabriëls I, Vergauwen L, de Boevre M, et al. Optimizing the use of zebrafish feeding trials for the safety evaluation of genetically modified crops[J]. International Journal of Molecular Sciences, 2019, 20(6):1472

    Google Scholar Pub Med

    Rayan A M, Nigussie F, Abbott L C. Safety evaluation of stacked genetically modified corn event (MON89034×MON88017) using zebrafish as an animal model[J]. Food and Nutrition Sciences, 2015, 6(14):1285-1295

    Google Scholar Pub Med

    National Research Council. Nutrient requirement of fish[R]. Washington DC:National Academy Press, 1993

    Google Scholar Pub Med

    董姗姗, 章嫡妮, 张振华, 等. 转mCry1Ac基因玉米BT799对斑马鱼的生态毒理学效应[J]. 应用生态学报, 2019, 30(8):2845-2853 Dong S S, Zhang D N, Zhang Z H, et al. Ecotoxicological effects of transgenic mCry1Ac maize (BT799) on zebrafish[J]. Chinese Journal of Applied Ecology, 2019, 30(8):2845-2853(in Chinese)

    Google Scholar Pub Med

    中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会. 饲料卫生标准:GB 13078-2017[S]. 北京:中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会, 2017 General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of China. Hygienical standard for feeds:GB 13078-2017[S]. Beijing:General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of China, 2017 (in Chinese)

    Google Scholar Pub Med

    Sanden M, Krogdahl A, Bakke-Mckellep A M, et al. Growth performance and organ development in Atlantic salmon, Salmo salar L. parr fed genetically modified (GM) soybean and maize[J]. Aquaculture Nutrition, 2006, 12(1):1-14

    Google Scholar Pub Med

    Sissener N H, Johannessen L E, Hevrøy E M, et al. Zebrafish (Danio rerio) as a model for investigating the safety of GM feed ingredients (soya and maize); performance, stress response and uptake of dietary DNA sequences[J]. The British Journal of Nutrition, 2010, 103(1):3-15

    Google Scholar Pub Med

    Nordgarden U, Oppedal F, Taranger G L, et al. Seasonally changing metabolism in Atlantic salmon (Salmo salar L.) I-Growth and feed conversion ratio[J]. Aquaculture Nutrition, 2003, 9(5):287-293

    Google Scholar Pub Med

    Sissener N H, Sanden M, Bakke A M, et al. A long term trial with Atlantic salmon (Salmo salar L.) fed genetically modified soy; focusing general health and performance before, during and after the parr-smolt transformation[J]. Aquaculture, 2009, 294(1-2):108-117

    Google Scholar Pub Med

    Sagstad A, Sanden M, Krogdahl, et al. Organs development, gene expression and health of Atlantic salmon (Salmo salar L.) fed genetically modified soybeans compared to the near-isogenic non-modified parental line[J]. Aquaculture Nutrition, 2008, 14(6):556-572

    Google Scholar Pub Med

    Sissener N H, Bakke A M, Gu J, et al. An assessment of organ and intestinal histomorphology and cellular stress response in Atlantic salmon (Salmo salar L.) fed genetically modified Roundup Ready® soy[J]. Aquaculture, 2009, 298(1-2):101-110

    Google Scholar Pub Med

    Sanden M, Ornsrud R, Sissener N H, et al. Cross-generational feeding of Bt (Bacillus thuringiensis)-maize to zebrafish (Danio rerio) showed no adverse effects on the parental or offspring generations[J]. The British Journal of Nutrition, 2013, 110(12):2222-2233

    Google Scholar Pub Med

    Goolish E M, Okutake K, Lesure S. Growth and survivorship of larval zebrafish Danio rerio on processed diets[J]. North American Journal of Aquaculture, 1999, 61(3):189-198

    Google Scholar Pub Med

    Sanden M, Jørgensen S, Hemre G I, et al. Zebrafish (Danio rerio) as a model for investigating dietary toxic effects of deoxynivalenol contamination in aquaculture feeds[J]. Food and Chemical Toxicology, 2012, 50(12):4441-4448

    Google Scholar Pub Med

    Iwashita Y, Yamamoto T, Furuita H, et al. Influence of certain soybean antinutritional factors supplemented to a casein-based semipurified diet on intestinal and liver morphology in fingerling rainbow trout Oncorhynchus mykiss[J]. Fisheries Science, 2008, 74(5):1075-1082

    Google Scholar Pub Med

    Nordrum S, Bakke-Mckellep A M, Krogdahl Å, et al. Effects of soybean meal and salinity on intestinal transport of nutrients in Atlantic salmon (Salmo salar L.) and rainbow trout (Oncorhynchus mykiss)[J]. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 2000, 125(3):317-335

    Google Scholar Pub Med

    Duke S O, Rimando A M, Pace P F, et al. Isoflavone, glyphosate, and aminomethylphosphonic acid levels in seeds of glyphosate-treated, glyphosate-resistant soybean[J]. Journal of Agricultural and Food Chemistry, 2003, 51(1):340-344

    Google Scholar Pub Med

    王德鑫. 黄豆苷元对尼罗罗非鱼(Oreochromis niloticus)生长的影响及其机制研究[D]. 青岛:中国海洋大学, 2014:5-7 Wang D X. The research of effects and mechanisms of dietary daidzein on the growth of Nile tilapia (Oreochromis niloticus)[D]. Qingdao:Ocean University of China, 2014:5 -7(in Chinese)

    Google Scholar Pub Med

    Bennetau-Pelissero C, Breton B B, Bennetau B, et al. Effect of genistein-enriched diets on the endocrine process of gametogenesis and on reproduction efficiency of the rainbow trout Oncorhynchus mykiss[J]. General and Comparative Endocrinology, 2001, 121(2):173-187

    Google Scholar Pub Med

    Pietsch C, Katzenback B A, Garcia-Garcia E, et al. Acute and subchronic effects on immune responses of carp (Cyprinus carpio L.) after exposure to deoxynivalenol (DON) in feed[J]. Mycotoxin Research, 2015, 31(3):151-164

    Google Scholar Pub Med

    Ryerse I A, Hooft J M, Bureau D P, et al. Diets containing corn naturally contaminated with deoxynivalenol reduces the susceptibility of rainbow trout (Oncorhynchus mykiss) to experimental Flavobacterium psychrophiluminfection[J]. Aquaculture Research, 2016, 47(3):787-796

    Google Scholar Pub Med

    Anater A, Manyes L, Meca G, et al. Mycotoxins and their consequences in aquaculture:A review[J]. Aquaculture, 2016, 451:1-10

    Google Scholar Pub Med

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Ecotoxicological Effects of Transgenic Cry1Ie Maize and Transgenic CP4-EPSPS Soybean on Zebrafish

  • Received Date: 15/12/2020
Fund Project:

Abstract: To evaluate the ecotoxicological effects of transgenic Cry1Ie insect-resistant maize and transgenic CP4-EPSPS herbicide-tolerant soybean on fish, zebrafish (Danio rerio) was used as a model animal and four experimental diets, including genetically modified (GM) maize, GM soybean, and corresponding near-isogenic non-GM lines were prepared respectively. A commercial feed was used as a reference diet. The feed intake, growth, reproduction, and antioxidant enzyme activity were investigated during a 98-day feeding trial. The histopathology and mRNA expression levels of sensitive protein were analyzed at different feeding stages. The results revealed that insect-resistant GM maize and herbicide-resistant GM soybean had no significant effects on growth performance, histopathology of the liver, intestinal tract, and brain, fecundity, and hatching rate of fertilized eggs. The activity of superoxide dismutase (SOD) was significantly higher in the liver of zebrafish fed with GM soybean compared with in those fed with the non-GM soybean and commercial diets, and was higher in male than in female fish (P<0.05). There were no obvious regular changes over time of mRNA expression levels of sensitive protein in zebrafish organ tissues. There were significant interaction effects between feeding duration, diet, and sex. When comparing experimental diets and commercial feed, we detected significant differences in growth performance, SOD activity in the liver, and mRNA expression levels, which may be related to the differences in palatability and nutrient composition of diets. In general, transgenic Cry1Ie insect-resistant maize and transgenic CP4-EPSPS herbicide-tolerant soybean have no obvious ecotoxicological effects on zebrafish.

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