2020 Volume 15 Issue 4
Article Contents
Previous Article Next Article

Liu Qingchun, Hua Chunxiu, Xue Shipeng, Guan Cuicui, Xia Xichao, Dai Hongmei, Wang Wen, Zhang Ke, Yao Lunguang. Cloning of AwCAT from Anodonta woodiana and the Effect of PBDE on Its Transcription[J]. Asian Journal of Ecotoxicology, 2020, 15(4): 203-214. doi: 10.7524/AJE.1673-5897.20191008002
Citation: Liu Qingchun, Hua Chunxiu, Xue Shipeng, Guan Cuicui, Xia Xichao, Dai Hongmei, Wang Wen, Zhang Ke, Yao Lunguang. Cloning of AwCAT from Anodonta woodiana and the Effect of PBDE on Its Transcription[J]. Asian Journal of Ecotoxicology, 2020, 15(4): 203-214. doi: 10.7524/AJE.1673-5897.20191008002
shu

Cloning of AwCAT from Anodonta woodiana and the Effect of PBDE on Its Transcription

  • 1. School of Basic Medicine, Nanyang Medical College, Nanyang 473007, China;

  • 2. School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China;

  • 3. College of Medicine, Pingdingshan University, Pingdingshan 476000, China

  • Catalase (CAT) is a major reactive oxygen species (ROS) scavenger enzyme that plays a significant role in the antioxidant defense mechanism of organisms by reducing toxic hydrogen peroxide molecules into a nontoxic form of oxygen and water with a high turnover rate. Polybrominated diphenyl ethers (PBDEs) are currently important components in flame retardants. Excess PBDEs deposited in organism could catalyze the production of ROS. In current study, one complete CAT sequence was isolated from Anodonta woodiana and named AwCAT . The full-length AwCAT cDNA of A. woodiana consists of 1 784 nucleotides. The open reading frame contains 1 536 bp nucleotides and encodes 512 amino acids. Compared with that of control group, AwCAT mRNA level of hepatopancreas increased more than 66.66% (P<0.05), 1.35 times (P<0.05), 1.54 times (P<0.05), 1.97 times (P<0.01) and 2.39 times (P<0.01) in the 6.25, 12.5, 25, 50 and 100 μg·L-1 of PBDE-47 treated groups, respectively; AwCAT mRNA level of hepatopancreas increased more than 7.84%, 35.38%, 61.53% (P<0.05), 1.03 times (P<0.05) and 1.09 times (P<0.05) in the 10, 20, 40, 80 and 160 μg·L-1 of PBDE-209 treated group, respectively. Compared with that of control group, AwCAT mRNA level of gill significantly increased in the PBDE-47 treated groups. Compared with that of control group, AwCAT mRNA level of gill increased more than 85.71% (P<0.05) in the PBDE-209 treated goups. These results indicate that up-regulations of AwCAT expression of hepatopancreas and gill in the freshwater bivalve A. woodiana are contributed to eliminate stress derived from PBDE-47 and PBDE-209 challenge.
  • 加载中
  • Longo V, Longo A, Di Sano C, et al. In vitro exposure to 2,2',4,4'-tetrabromodiphenyl ether (PBDE-47) impairs innate inflammatory response[J]. Chemosphere, 2019, 219:845-854

    Google Scholar Pub Med

    Fromme H, Becher G, Hilger B, et al. Brominated flame retardants-Exposure and risk assessment for the general population[J]. International Journal of Hygiene and Environmental Health, 2016, 219:1-23

    Google Scholar Pub Med

    Zhang R, Guo J, Wu F, et al. Toxicity reference values for polybrominated diphenyl ethers:Risk assessment for predatory birds and mammals from two Chinese lakes[J]. Reviews of Environmental Contamination and Toxicology, 2014, 229:111-137

    Google Scholar Pub Med

    Cruz R, Cunha S C, Casal S. Brominated flame retardants and seafood safety:A review[J]. Environment International, 2015, 77:116-131

    Google Scholar Pub Med

    Zezza D, Tait S, Della Salda L, et al. Toxicological, gene expression and histopathological evaluations of environmentally realistic concentrations of polybrominated diphenyl ethers PBDE- 47, PBDE-99 and PBDE-209 on zebrafish embryos[J]. Ecotoxicology and Environmental Safety, 2019, 183:109566

    Google Scholar Pub Med

    Sellaththurai S, Priyathilaka T T, Lee J. Molecular cloning, characterization, and expression level analysis of a marine teleost homolog of catalase from big belly seahorse (Hippocampus abdominalis)[J]. Fish Shellfish Immunology, 2019, 89:647-659

    Google Scholar Pub Med

    Zhang H C, Ma K X, Yang Y J, et al. Molecular cloning, characterization, expression and enzyme activity of catalase from planarian Dugesia japonica in response to environmental pollutants[J]. Ecotoxicology and Environmental Safety, 2018, 165:88-95

    Google Scholar Pub Med

    Kim U J, Jo H, Lee I S, et al. Investigation of bioaccumulation and biotransformation of polybrominated diphenyl ethers, hydroxylated and methoxylated derivatives in varying trophic level freshwater fishes[J]. Chemosphere, 2015, 137:108-114

    Google Scholar Pub Med

    Soo P, Todd P A. The behaviour of giant clams (Bivalvia:Cardiidae:Tridacninae)[J]. Marine Biology, 2014, 161(12):2699-2717

    Google Scholar Pub Med

    Xia X, Yu R, Li M, et al. Molecular cloning and characterization of two genes encoding peroxiredoxins from freshwater bivalve Anodonta woodiana:Antioxidative effect and immune defense[J]. Fish Shellfish Immunology, 2018, 82:476-491

    Google Scholar Pub Med

    Xia X, Xue S, Wang X, et al. Response a chronic effects of PBDE-47:Up-regulations of HSP60 and HSP70 expression in freshwater bivalve Anodonta woodiana[J]. Fish Shellfish Immunology, 2017, 65:213-225

    Google Scholar Pub Med

    Bigot A, Vasseur P, Rodius F. SOD and CAT cDNA cloning, and expression pattern of detoxification genes in the freshwater bivalve Unio tumidus transplanted into the Moselle River[J]. Ecotoxicology, 2010, 19:369-376

    Google Scholar Pub Med

    Freire A C G, Alves C L, Goes G R, et al. Catalase expression impairs oxidative stress-mediated signalling in Trypanosoma cruzi[J]. Parasitology, 2017, 144(11):1498-1510

    Google Scholar Pub Med

    Lin H, Dai R, Li J, et al. The mechanisms of hippocampal neurons exposed to PBDE-209 induce oxidative stress and apoptosis[J]. Journal of Hygiene Research, 2016, 45(6):977-983

    Google Scholar Pub Med

    Danková M, Domoráková I, Fagová Z, et al. Bradykinin and noradrenaline preconditioning influences level of antioxidant enzymes SOD, CuZn-SOD, Mn-SOD and catalase in the white matter of spinal cord in rabbits after ischemia/reperfusion[J]. European Journal of Histochemistry, 2019, 63(4):197

    Google Scholar Pub Med

    Xu X, You K, Bu R. Proximal tubular development is impaired with downregulation of MAPK/ERK signaling, HIF-1α, and catalase by hyperoxia exposure in neonatal rats[J]. Oxidative Medicine and Cellular Longevity, 2019(4):1-16

    Google Scholar Pub Med

    Davies R, Zou E. Polybrominated diphenyl ethers disrupt molting in neonatal Daphnia magna[J]. Ecotoxicology, 2012, 21:1371-1380

    Google Scholar Pub Med

    张曼, 苏永全, 冯文荣, 等. 日本囊对虾过氧化氢酶cDNA全长克隆及表达分析[J]. 厦门大学学报, 2013, 52(6):851-858 Zhang M, Su Y Q, Feng W R, et al. Cloning and expression of catalase cDNA from Penaeus japonicus[J]. Journal of Xiamen University, 2013, 52(6):851-858(in Chinese)

    Google Scholar Pub Med

    成嘉, 符贵红, 刘芳, 等. 重金属铅对鲫鱼乳酸脱氢酶和过氧化氢酶活性的影响[J]. 生命科学研究, 2016, 10(4):372-376 Cheng J, Fu G H, Liu F, et al. Effects of heavy metal lead on activities of LDH and CAT in Carassius auratus[J]. Life Science Research, 2016, 10(4):372-376(in Chinese)

    Google Scholar Pub Med

    高层. 重金属Pb2+和Cu2+对太平洋牡蛎(Crassostrea gigas)毒性效应的比较研究[D]. 青岛:中国海洋大学, 2012:35-39 Gao C. A comparative study on the toxic effects of heavy metals Pb2+ and Cu2 + on Pacific oyster[D]. Qingdao:Ocean University of China, 2012:35-39(in Chinese)

    Google Scholar Pub Med

    Prasad A, Pospíšil P, Tada M. Editorial:Reactive oxygen species (ROS) detection methods in biological system[J]. Frontiers in Physiology, 2019, 10:1316

    Google Scholar Pub Med

    de Andrade K Q, Moura F A, Dos Santos J M, et al. Oxidative stress and inflammation in hepatic diseases:Therapeutic possibilities of N-acetylcysteine[J]. International Journal of Molecular Sciences, 2015, 16:30269-30308

    Google Scholar Pub Med

    Hallmann A, Konieczna L, Swiezak J, et al. Aromatisation of steroids in the bivalve Mytilus trossulus[J]. The Journal of Life and Environmental Sciences, 2019, 7:e6953

    Google Scholar Pub Med

  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Export Citation
PDF
XML

Article Metrics

Article views(1187) PDF downloads(19) Cited by(0)

Access History

Cloning of AwCAT from Anodonta woodiana and the Effect of PBDE on Its Transcription

  • Received Date: 08/10/2019
Fund Project:

Abstract: Catalase (CAT) is a major reactive oxygen species (ROS) scavenger enzyme that plays a significant role in the antioxidant defense mechanism of organisms by reducing toxic hydrogen peroxide molecules into a nontoxic form of oxygen and water with a high turnover rate. Polybrominated diphenyl ethers (PBDEs) are currently important components in flame retardants. Excess PBDEs deposited in organism could catalyze the production of ROS. In current study, one complete CAT sequence was isolated from Anodonta woodiana and named AwCAT . The full-length AwCAT cDNA of A. woodiana consists of 1 784 nucleotides. The open reading frame contains 1 536 bp nucleotides and encodes 512 amino acids. Compared with that of control group, AwCAT mRNA level of hepatopancreas increased more than 66.66% (P<0.05), 1.35 times (P<0.05), 1.54 times (P<0.05), 1.97 times (P<0.01) and 2.39 times (P<0.01) in the 6.25, 12.5, 25, 50 and 100 μg·L-1 of PBDE-47 treated groups, respectively; AwCAT mRNA level of hepatopancreas increased more than 7.84%, 35.38%, 61.53% (P<0.05), 1.03 times (P<0.05) and 1.09 times (P<0.05) in the 10, 20, 40, 80 and 160 μg·L-1 of PBDE-209 treated group, respectively. Compared with that of control group, AwCAT mRNA level of gill significantly increased in the PBDE-47 treated groups. Compared with that of control group, AwCAT mRNA level of gill increased more than 85.71% (P<0.05) in the PBDE-209 treated goups. These results indicate that up-regulations of AwCAT expression of hepatopancreas and gill in the freshwater bivalve A. woodiana are contributed to eliminate stress derived from PBDE-47 and PBDE-209 challenge.

    HTML

Reference (23)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return

    All rights reserved: Reesearch Center for Eco-Environmental Sciences,Chinese Academy of Sciences Copyright © 1997-2016

    Address:  Postcode: 100085 Phone: 010-62941072 E-mail: stdlxb@rcees.ac.cn

    Supported by: Beijing Renhe Information Technology Co. Ltd  Technical support: info@rhhz.net