心脏内膜是苯并芘与α-萘黄酮的毒性靶点？
作者简介:
杨景峰(1974-),男,副教授,博士,研究方向为鱼类生物技术,Email:yangjf@188.com
- 1. 内蒙古民族大学动物科技学院/内蒙古自治区高等学校毒物与动物疾病监控重点实验室, 通辽 028000;
- 2. 内蒙古民族大学农学院, 通辽 028000;
- 3. 内蒙古民族大学生命科学院, 通辽 028000;
- 4. 路易斯维尔百奥生生物科技公司, 美国德克萨斯州;
- 5. 军事医学科学院毒物药物研究所, 北京 100850
摘要: 苯并芘(benzo-a-pyrene,BaP)是一种典型的致癌物质，具有一定的心血管毒性。试验在形态学观察的基础上，检测了细胞色素P450(cyp1A)在心脏的表达。结果表明虽然α-萘黄酮(ANF)没有诱导，但BaP和BaP+ANF强烈诱导cyp1A的基因表达，以及在心脏内膜有强烈的蛋白质表达，在BaP与ANF同时染毒引起心脏水肿。说明心脏是BaP以及ANF的目标组织，进一步说明鱼胚胎在药物以及环境物质评价中的优势。
Heart is the Target Organ of BaP and Alpha Naphthyl Flavonoids？
- 1. Inner Mongolia Provincial Key Laboratory for Toxicants and Animal Disease, College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China;
- 2. College of Agriculture Science, Inner Mongolia University for Nationalities, Tongliao 028000, China;
- 3. School of Life Science, Inner Mongolia University for Nationalities, Tongliao 028000, China;
- 4. Bio-Synthesis, Inc. -DNA Lab Department, Lewisville, TX, USA;
- 5. Institute of Pharmacology and Toxicology, Academy of Military Medical Science, Beijing 100850, China
- Received Date:
2015-09-25
Fund Project:
Abstract: Benzo-a-pyrene (BaP) is a typical carcinogen, and has cardiovascular toxicity. We focused on heart morphological changes, cytochrome P4501A mRNA (cyp1A) expression and protein expression. In the heart, although the results showed that α-naphthoflavone (ANF) did not induce the expression of cyp1A mRNA, but cyp1A mRNA was strongly induced by BaP and BaP + ANF treatment, and cyp1A protein expression was also strongly induced in the inner membranes of heart. The results indicate that the heart is a target tissue of BaP and ANF, further illustrating the advantages of fish embryos for the evaluation of drugs and environmental substances.
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