新型阻燃剂TCPP对斑马鱼的毒性研究

皮天星; 蔡磊明; 蒋金花; 赵学平; 王彦华; 吴声敢; 苏连水; 汤涛

doi:10.7524/AJE.1673-5897.20150709002

新型阻燃剂TCPP对斑马鱼的毒性研究

1. 新疆农业大学草业与环境科学学院, 乌鲁木齐 830052;

2. 浙江省农业科学院农产品质量标准研究所浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地农业部农药残留检测重点实验室, 杭州 310021

作者简介: 皮天星(1989-),男,硕士研究生,研究方向为环境毒理学,E-mail:ptx@163.com

通讯作者: 蔡磊明, E-mail: hzcaileiming@foxmail.com ;

基金项目:

农药助剂环境风险评估及管理研究(2013467026)

中图分类号: X171.5

Toxicity Effects of A New Flame Retardant Tris(2-chloroisopropyl) Phosphate to Zebrafish (Danio rerio)

1. College of Grassland and Environment Sciences, Xinjiang Agricultural University, Urumqi 830052, China;

2. State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Key Laboratory for Pesticide Residue Detection of Minsitry of Agriculture, Institute of Quality and Standard of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Corresponding author: Cai Leiming, hzcaileiming@foxmail.com ;

Fund Project:

摘要: 为了明确新型阻燃剂磷酸三(2-氯丙基)酯(TCPP)的生态风险，本研究采用斑马鱼为模式生物，评价了TCPP对成鱼和胚胎的毒性效应。急性毒性研究结果表明，TCPP对斑马鱼成鱼的96 h致死中浓度(LC₅₀)为47.06 mg·L^-1，而对胚胎96 h-LC₅₀为26.01 mg·L^-1，且会影响胚胎的正常发育，导致孵化出的仔鱼产生畸形。成鱼14 d延长毒性试验结果表明，TCPP对斑马鱼成鱼的无可观察效应浓度(NOEC)为1.00 mg·L^-1，染毒暴露后肝脏和性腺指数随TCPP浓度增加轻微下降，但肝脏中卵黄蛋白原(VTG)的含量和性腺中芳香化酶的活性随TCPP浓度增加普遍升高。此外，TCPP的暴露还会导致斑马鱼脑垂体中合成促性腺激素的相关基因表达量增加。因此，TCPP对斑马鱼成鱼和胚胎的急性毒性均为低毒级，但长期暴露会干扰内分泌系统的调控功能，影响斑马鱼的正常发育。

Abstract: To understand the ecological risk of fire retardant tris(2-chloropropyl) phosphate (TCPP), we investigated its toxic effects on adult zebrafish and zebrafish embryos. Acute toxicological study indicated that the LC₅₀ of TCPP for adult zebrafish in 96 h was 47.06 mg·L^-1, and that for zebrafish embryos in 96 h was 26.01 mg·L^-1. Moreover, TCPP could impair the embryo development, leading to deformed larvae. The 14-day chronic toxicity study indicated that the no observed effect concentration (NOEC) of TCPP towards adult zebrafish was 1.00 mg·L^-1. The liver and gonad function index slightly declined with the chronic exposure of increased TCPP concentration. However, the levels of liver vitellogenin (VTG) and gonadal aromatase were increased. In addition, exposure to TCPP resulted in the increased expression of the genes involved in the biosynthesis of gonadotropin in the pituitary. Therefore, the acute toxicity of TCPP to adult zebrafish and its embryos was low toxicity grade. However, chronic exposure disrupted the endocrine system and affected the normal development of zebrafish.

Key words:

tris (2-chloroisopropyl) phosphate (TCPP) /
zebrafish (Danio rerio) /
developmental toxicity

新型阻燃剂TCPP对斑马鱼的毒性研究

通讯作者: 蔡磊明, E-mail: hzcaileiming@foxmail.com ;

作者简介: 皮天星(1989-),男,硕士研究生,研究方向为环境毒理学,E-mail:ptx@163.com
1. 新疆农业大学草业与环境科学学院, 乌鲁木齐 830052;

2. 浙江省农业科学院农产品质量标准研究所浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地农业部农药残留检测重点实验室, 杭州 310021

收稿日期: 2015-07-09

基金项目:

农药助剂环境风险评估及管理研究(2013467026)

关键词:

Toxicity Effects of A New Flame Retardant Tris(2-chloroisopropyl) Phosphate to Zebrafish (Danio rerio)

Corresponding author: Cai Leiming, hzcaileiming@foxmail.com ;

1. College of Grassland and Environment Sciences, Xinjiang Agricultural University, Urumqi 830052, China;

Received Date: 2015-07-09

Fund Project:

Keywords:

tris (2-chloroisopropyl) phosphate (TCPP) /
zebrafish (Danio rerio) /
developmental toxicity

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