摘要:
为探明三唑酮对鱼类不同生长阶段的毒性效应,以斑马鱼和稀有鮈鲫为测试生物,检测了三唑酮对斑马鱼和稀有鮈鲫不同生长阶段的急性毒性差异。研究发现,三唑酮对斑马鱼和稀有鮈鲫胚胎为低毒,其96 h-LC50值分别为21.1(14.431.0)和14.2(9.6520.9)mg·L-1;高浓度的三唑酮对斑马鱼和稀有鮈鲫胚胎的孵化有明显的抑制作用,染毒96 h后,23.7 mg·L-1三唑酮组斑马鱼胚胎的孵化率为4.17%,22.5 mg·L-1三唑酮组稀有鮈鲫胚胎的孵化率为33.3%。三唑酮对斑马鱼仔鱼、幼鱼和成鱼为低毒,其96 h-LC50值分别为24.8、21.3、13.1 mg·L-1,三唑酮对稀有鮈鲫仔鱼、幼鱼和成鱼为中毒,其96 h-LC50值分别为9.96、7.89、6.89 mg·L-1。因此,三唑酮对斑马鱼和稀有鮈鲫不同生长阶段的毒性效应排序一致,毒性从高到低顺序依次为:成鱼 > 幼鱼 > 仔鱼。试验结果表明,相对于斑马鱼,稀有鮈鲫对三唑酮的毒性作用更为敏感,三唑酮对稀有鮈鲫不同生长阶段的毒性均高于斑马鱼相应生长阶段的毒性。
关键词:
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三唑酮
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斑马鱼
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稀有鮈鲫
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急性毒性
Abstract:
To explore the toxic effects of triadimefon on fish at different life stages, the differences in acute toxicity of triadimefon to zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) at different life stages were evaluated. The results showed that triadimefon was low toxic to the embryos of both zebrafish and rare minnow, and the 96 h-LC50 value was 21.1 (14.4~31.0) and 14.2 (9.65~20.9) mg·L-1, respectively. An obvious inhibition of embryos hatching for both fish was observed after the exposure to high concentration of triadimefon for 96 h, and the hatching rate was 4.17% and 33.3% when the embryos of zebrafish and rare minnow were exposed to 23.7 and 22.5 mg·L-1 triadimefon, respectively. Triadimefon exhibits low toxic to the larvae, juvenile, and adult of zebrafish with the 96 h-LC50 values of 24.8, 21.3, and 13.1 mg·L-1 respectively. Triadimefon shows moderately toxic to the larvae, juvenile, and adult of rare minnow with the 96 h-LC50 values of 9.96, 7.89, and 6.89 mg·L-1. The trend of triadimefon acute toxicity to zebrafish and rare minnow at different life stages was the same:adult>juvenile>larva, and rare minnow was more sensitive to triadimefon during different life stages compared with zebrafish.
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