首页 | 期刊简介 | 编委会 | 投稿要求 | 化学品管理信息动态 | 学术会议 | 专辑 | 毒理学测试技术设备 | 订购本刊 | 联系我们 | English version

王法琦,刘薇,金一和,马军胜,等. 胚胎期及哺乳期全氟辛烷磺酸盐(PFOS)暴露对大鼠长时程突触可塑性影响的miRNA组学研究[J]. 生态毒理学报, 2012, 7(5): 491-500
胚胎期及哺乳期全氟辛烷磺酸盐(PFOS)暴露对大鼠长时程突触可塑性影响的miRNA组学研究
Exploration of the Potential Effect of Perfluorooctane Sulfonate (PFOS) Neonatal Exposure on Long-term Potentiation during Synaptic Transimission by MiRNA Microarray
投稿时间:2011-11-17  修订日期:2012-01-11
DOI:
中文关键词:  全氟辛烷磺酸盐  长时程增强效应  miRNA芯片  突触可塑性
英文关键词:perfluorooctane sulfonate  LTP  miRNA microarray  synaptic plasticity
基金项目:国家自然科学基金项目(21177020, 20837004)
作者单位
王法琦 大连理工大学环境与生命科学技术学院 教育部工业生态和环境工程重点实验室大连 116024 
刘薇 大连理工大学环境与生命科学技术学院 教育部工业生态和环境工程重点实验室大连 116024 
金一和 大连理工大学环境与生命科学技术学院 教育部工业生态和环境工程重点实验室大连 116024 
马军胜 大连理工大学环境与生命科学技术学院 教育部工业生态和环境工程重点实验室大连 116024 
于明曦 大连理工大学环境与生命科学技术学院 教育部工业生态和环境工程重点实验室大连 116024 
摘要点击次数: 2578
全文下载次数: 1164
中文摘要:
      为探讨PFOS胚胎期及哺乳期暴露对动物子代学习记忆能力影响的分子机理,采用微小RNA(miRNA)芯片技术检测PFOS胚胎期及哺乳期暴露对出生第1和7天大鼠脑组织miRNA表达的影响,分析突触可塑性相关miRNA表达的差异变化。结果显示,经PFOS暴露后出生第1和7天的大鼠脑组织中分别有24和17个miRNA发生显著性差异表达(p<0.05),其中与突触传递和神经递质转运等相关的miRNA的差异表达最为显著,主要包括miR-466b、miR-672、miR-297、miR-674-3p和miR-207。差异表达miRNA的路径分析显示出生后1和7 d的大鼠的长时程增强效应(LTP)均受PFOS显著影响(p<0.05),这说明PFOS胚胎期及哺乳期暴露可能通过影响LTP的形成、发展和维持过程对大鼠子代大脑学习记忆能力造成威胁,并且miR-466b、miR-672、miR-297、miR-674-3p和miR-207可能参与了其中的调控过程。
              
AuthorAffiliation
Wang FaqiKey Laboratory of Industrial Ecology and Environmental Engineering of Ministry of Education, School of Environmental and Biological Science and Technology, , Dalian University of Technology, Dalian 116024, China
Liu WeiKey Laboratory of Industrial Ecology and Environmental Engineering of Ministry of Education, School of Environmental and Biological Science and Technology, , Dalian University of Technology, Dalian 116024, China
Jin YiheKey Laboratory of Industrial Ecology and Environmental Engineering of Ministry of Education, School of Environmental and Biological Science and Technology, , Dalian University of Technology, Dalian 116024, China
Ma JunshengKey Laboratory of Industrial Ecology and Environmental Engineering of Ministry of Education, School of Environmental and Biological Science and Technology, , Dalian University of Technology, Dalian 116024, China
Yu MingxiKey Laboratory of Industrial Ecology and Environmental Engineering of Ministry of Education, School of Environmental and Biological Science and Technology, , Dalian University of Technology, Dalian 116024, China
英文摘要:
      To explore the molecular mechanism underlying the toxic effect of PFOS prenatal and neonatal exposure on the study and memory ability of offspring, miRNA arrays were used to profile the expression of brain miRNAs in neonatal rats on postnatal day (PND) 1 and 7 with prenatal and neonatal exposure of PFOS. The results showed that twenty-four brain miRNAs on PND 1 and seventeen on PND 7 rats were significantly changed after PFOS exposure (p< 0.05). miR-466b, miR-672, miR-297, miR-674-3p and miR-207, which participate in neurotransmitter transport and synaptic transmission, showed the highest differential expression. The analysis of pathways associated with differentially expressed miRNAs indicated that long-term potentiation (LTP) in rat pups on PND 1 and 7 rats were significantly affected by PFOS exposure (p<0.05). It is demonstrated that prenatal and neonatal exposure of PFOS could threaten the study and memory ability of rat offspring through the effect of the formation, maturation and maintenance of LTP. Moreover, miR-466b, miR-672, miR-297, miR-674-3p and miR-207 might be involved in the above process.
查看全文  查看/发表评论  下载PDF阅读器
关闭

您是第5264176位访问者   京ICP备 09058833 号

主办单位:中国科学院生态环境研究中心     单位地址:北京市海淀区双清路18号

 服务热线:010-62941072       传真:010-62923563       邮编:100085    Email: stdlxb@rcees.ac.cn

    本系统由北京勤云科技发展有限公司设计

0463美女美女3764美女美女ktv