白洋淀典型抗生素与底栖藻类群落指标相关性研究
The Correlation between Periphyton Community Indicator and Typical Antibiotics in Baiyangdian Lake
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摘要: 底栖藻类作为湖泊中主要的生产者，其对抗生素较为敏感，目前有关喹诺酮类抗生素(quinolones, QNs)与底栖藻类群落的相关性研究较为缺乏，因此本研究选取白洋淀为研究区，利用超高效液相色谱串联质谱法(HPLC-MS/MS)检测水体QNs浓度，并分析底栖藻类群落结构和功能指标，利用商值法(RQ)计算QNs的生态风险值，建立QNs生态风险与底栖藻类群落指标的相关性。研究结果如下。(1) 白洋淀QNs浓度存在明显时空差异。就空间分布而言，QNs的最高浓度出现在生境1(1 309.80 ng·L-1)；就时间变化而言，4月QNs浓度最高；就QNs种类而言，氟甲喹(flumequine, FLU)浓度最高(1 054.38 ng·L-1)；(2) 就藻类群落指标的空间分布而言，除藻密度(AD)、叶绿素a(Chl a)、叶绿素b(Chl b)、叶绿素b/a(Chl b/a)、绿藻比例(CHL)、蓝藻比例(CYA)、碱性磷酸酶(APA)、β-葡萄糖苷酶(GLU)、亮氨酸氨基肽酶(LEU)和无灰干重(AFDW)等指标最大值出现在生境2外，其他指标最大值出现在生境3；就时间分布而言，大部分底栖藻类群落指标11月值高于4月和8月；(3) 除FLU的生态风险处于中等水平外，其余QNs生态风险较低；其中，8月生境2的生态风险值最高(RQ最大值为0.9446)；(4) AD、Chl a、Chl b、叶绿素c(Chl c)、Chl b/a与RQCIP和RQFLU呈显著相关，其中Chl a与RQFLU的相关性显著(r = 0.827，P<0.01)。结果表明，底栖藻类结构指标与QNs风险值相关性较为显著，因此，可考虑筛选较为敏感的底栖藻类群落结构指标，为富营养化湖泊生态监测方法研究提供理论基础及相关数据支撑。Abstract: Periphyton, the main producer in lakes, are sensitive to antibiotics. At present, the correlation between quinolones (QNs) and periphyton is relatively scarce. Therefore, Baiyangdian Lake was selected as the research area, and the concentration of QNs in water was detected by HPLC-MS/MS. The structure and function indicators of periphyton were analyzed, and the ecological risk values of QNs were calculated by entropy method (based on risk quotient, abbreviated to RQ). Finally, the correlation between ecological risk of QNs and periphyton community indexes was established. The results showed as follows. (1) There were obvious spatial and temporal differences in QNs concentrations in Baiyangdian Lake. In terms of spatial distribution, the highest concentration of QNs appeared in Habitat 1 (1 309.80 ng·L-1); for the aspect of seasonal variation, the highest concentration of QNs was in April; in terms of QNs type, the highest concentration of antibiotics was flumequine (FLU) (1 054.38 ng·L-1); (2) in terms of spatial distribution, except the values of algal density (AD), chlorophyll a (Chl a), chlorophyll b (Chl b), chlorophyll b/a (Chl b/a), the proportion of chlorophyta (CHL), the proportion of cyanophyta (CYA), alkaline phosphatase (APA), β-glucose glycosidase (GLU), leucine amino peptide enzymes (LEU) and ash-free dry weight (AFDW) were highest in Habitat 2, others were highest in Habitat 3. For the aspect of seasonal variation, most periphyton indicators in November were higher than those in April and August; (3) the value of ecological risks of FLU was the highest; the value of ecological risk was highest in habitat 2 in August (RQmax=0.9446). (4) AD, Chl a, Chl b, chlorophyll c (Chl c), Chl b/a were correlated with RQCIP and RQFLU, in which the correlation between Chl a and RQFLU was significant (r = 0.827, P<0.01). The results showed that the correlation between periphyton community indexes and ecological risk value of QNs were significant. Therefore, more sensitive periphyton community indexes could be selected to support the study of ecological monitoring methods of eutrophic lakes.
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Key words:
- quinolone /
- periphyton /
- ecological risk /
- structural and functional metrics /
- Baiyangdian Lake
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