石墨烯对高等植物幼苗的毒性及机理探究

徐亚楠; 徐立娜

doi:10.7524/AJE.1673-5897.20190711001

生态毒理学报

石墨烯对高等植物幼苗的毒性及机理探究

徐亚楠, 徐立娜. 石墨烯对高等植物幼苗的毒性及机理探究[J]. 生态毒理学报, 2020, 15(1): 220-229. doi: 10.7524/AJE.1673-5897.20190711001

引用本文:

徐亚楠, 徐立娜. 石墨烯对高等植物幼苗的毒性及机理探究[J]. 生态毒理学报, 2020, 15(1): 220-229. doi: 10.7524/AJE.1673-5897.20190711001

Xu Yanan, Xu Lina. Phytotoxicity of Graphene to Higher Plants’ Seedlings and Its Mechanisms[J]. Asian Journal of Ecotoxicology, 2020, 15(1): 220-229. doi: 10.7524/AJE.1673-5897.20190711001

Citation:

Xu Yanan, Xu Lina. Phytotoxicity of Graphene to Higher Plants’ Seedlings and Its Mechanisms[J]. Asian Journal of Ecotoxicology, 2020, 15(1): 220-229. doi: 10.7524/AJE.1673-5897.20190711001

石墨烯对高等植物幼苗的毒性及机理探究

徐亚楠¹,
徐立娜²

1. 中国海洋大学海洋环境与生态教育部重点实验室, 青岛 266100;
2. 青岛农业大学园林与林学院, 青岛 266100

作者简介: 徐亚楠(1989-),女,硕士研究生,研究方向为海洋环境生态学,E-mail:yanango@163.com

基金项目:
国家自然科学基金重大国际合作交流项目(41120134004)；青岛市博士后应用项目(861605040062)
中图分类号: X171.5

Phytotoxicity of Graphene to Higher Plants’ Seedlings and Its Mechanisms

Xu Yanan¹,
Xu Lina²

1. Ministry of Education Key Laboratory of Marine Environment and Ecology, Ocean University of China, Qingdao 266100, China;
2. College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao 266100, China
Fund Project:

摘要: 随着石墨烯产品的广泛应用和潜在的环境释放，其对生态环境的影响已引起广泛关注。为探讨石墨烯对高等植物生长的影响，探究了其对黄瓜幼苗和玉米幼苗生长的影响及其致毒机理。结果表明，水培条件下，不同浓度的石墨烯(10、50、100、500、1 000和2 000 mg·L^-1)处理植物幼苗15 d后，对植物幼苗的生长具有抑制作用。且随着处理时间和石墨烯浓度的增加，植物幼苗生长的所有指标，包括根/地上部鲜重和干重、根长、根尖数、株高和叶面积均相应降低。另外，黄瓜幼苗比玉米幼苗对石墨烯更加的敏感。进一步研究发现，石墨烯与黄瓜幼苗根部直接接触导致的物理损伤、氧化损伤，以及营养耗竭是其致毒机理。而石墨烯对玉米幼苗的致毒机理包括物理损伤和营养耗竭。本研究为石墨烯的环境风险评价提供了基础数据。
- 石墨烯 /
- 植物幼苗 /
- 植物毒性 /
- 物理损伤 /
- 营养耗竭
Abstract: With the extensive application and potential release of graphene materials, the negative effects of graphene have attracted great attention. To investigate the effects of graphene on the growth of higher plants, toxicity and related mechanisms of graphene on cucumber and maize seedlings were studied. After exposure to graphene (10, 50, 100, 500, 1 000 and 2 000 mg·L^-1) for 15 days under hydroponic conditions, the growth of the seedlings was inhibited. In addition, all the growth indexes, including root/shoot fresh weight and dry weight, root length, number of root tips, plant height and leaf area were also inhibited, and the negative effects were concentration and time-dependent. It was noted that cucumber seedlings were more sensitive to graphene than maize seedlings. Furthermore, physical damage, nutrient depletion, and oxidative stress were the main toxic mechanisms for graphene on cucumber seedlings. While, the toxicity mechanisms of graphene on maize seedlings were physical damage, and nutrient depletion. This study provided useful information for risk assessment of graphene in environment.
- graphene /
- plant seedlings /
- phytotoxicity /
- physical damage /
- nutrient depletion

Geim A K. Graphene:Status and prospects[J]. Science, 2009, 324(5934):1530-1534

Mittal G, Dhand V, Rhee K Y, et al. A review on carbon nanotubes and graphene as fillers in reinforced polymer nanocomposites[J]. Journal of Industrial and Engineering Chemistry, 2015, 21(2):11-25

Neto A H C, Guinea F, Peres N M R. The electronic properties of graphene[J]. Reviews of Modern Physics, 2009, 81(1):109

Luo Z, Zhou M, Weng J. Graphene-based passively Qswitched dual-wavelength erbium-doped fiber laser[J]. Optics Letters, 2010, 35(21):3709-3711

Zakharchenko K V, Los J H, Katsnelson M I, et al. Atomistic simulations of structural and thermodynamic properties of bilayer graphene[J]. Physical Review B, 2010, 81(23):235439

Mattevi C, Eda G, Agnoli S, et al. Evolution of electrical, chemical, and structural properties of transparent and conducting chemically derived graphene thin films[J]. Advanced Functional Materials, 2009, 19(16):2577-2583

Shareena T P D, McShan D, Dasmahapatra A K, et al. A review on graphene-based nanomaterials in biomedical applications and risks in environment and health[J]. Nanomicro Letters, 2018, 10(3):53

Xu J, Cao Z, Zhang Y, et al. A review of functionalized carbon nanotubes and graphene for heavy metal adsorption from water:Preparation, application, and mechanism[J]. Chemosphere, 2018, 195:351-364

Hu X, Zhou Q. Health and ecosystem risks of graphene[J]. Chemical Reviews, 2013, 113(5):3815-3835

Begum P, Ikhtiari R, Fugetsu B. Graphene phytotoxicity in the seedling stage of cabbage, tomato, red spinach, and lettuce[J]. Carbon, 2011, 49:3907-3919

Hao Y, Ma C, Zhang Z, et al. Carbon nanomaterials alter plant physiology and soil bacterial community composition in a rice-soil-bacterial ecosystem[J]. Environmental Pollution, 2018, 232:123-136

Zhang M, Gao B, Chen J. Effects of graphene on seed germination and seedling growth[J]. Journal of Nanoparticle Research, 2015, 17(2):78

Long Z, Ji J, Yang K, et al. Correction to systematic and quantitative investigation of the mechanism of carbon nanotubeśtoxicity toward algae[J]. Environmental Science and Technology, 2014, 48(8):4634

钟芬,潘雪江,刘恒全.改良氧化还原法制备石墨烯及其电学性能研究[J].化工新型材料, 2019, 47(4):67-70

Zhong F, Pan X J, Liu H Q. Preparation of graphene by modified redox and its electrical property[J]. New Chemical Materials, 2019, 47(4):67-70(in Chinese)

Wang Z Y, Xie X Y, Zhao J, et al. Xylem-and phloembased transport of CuO nanoparticles in maize (Zea mays L.)[J]. Environmental Science and Technology, 2012, 46(8):4434-4441

王发园.人工纳米颗粒的植物毒性及其在植物中的吸收和累积[J].生态毒理学报, 2012, 7(2):140-147

Wang F Y. Phytotoxicity of engineered nanoparticles (ENPs) and their uptake and accumulation in plants[J]. Asian Journal of Ecotoxicology, 2012, 7(2):140-147(in Chinese)

Zhao J, Wang Z Y, White J C, et al. Graphene in the aquatic environment:Adsorption, dispersion, toxicity and transformation[J]. Environmental Science and Technology, 2014, 48(17):9995-10009

Ganesan P, Kamaraj R, Vasudevan S. Application of isotherm, kinetic and thermodynamic models for the adsorption of nitrate ions on graphene from aqueous solution[J]. Journal of the Taiwan Institute of Chemical Engineers, 2013, 44(5):808-814

Zhao J, Cao X S, Wang Z Y, et al. Mechanistic understanding toward the toxicity of graphene-family materials to freshwater algae[J]. Water Research, 2017, 111:18-27

点击查看大图

计量

文章访问数: 2222
HTML全文浏览数: 2222
PDF下载数: 63
施引文献: 0

石墨烯对高等植物幼苗的毒性及机理探究

徐亚楠¹,
徐立娜²

作者简介: 徐亚楠(1989-),女,硕士研究生,研究方向为海洋环境生态学,E-mail:yanango@163.com
1. 中国海洋大学海洋环境与生态教育部重点实验室, 青岛 266100;

2. 青岛农业大学园林与林学院, 青岛 266100

收稿日期: 2019-07-11

基金项目:

国家自然科学基金重大国际合作交流项目(41120134004)；青岛市博士后应用项目(861605040062)

关键词:

摘要: 随着石墨烯产品的广泛应用和潜在的环境释放，其对生态环境的影响已引起广泛关注。为探讨石墨烯对高等植物生长的影响，探究了其对黄瓜幼苗和玉米幼苗生长的影响及其致毒机理。结果表明，水培条件下，不同浓度的石墨烯(10、50、100、500、1 000和2 000 mg·L^-1)处理植物幼苗15 d后，对植物幼苗的生长具有抑制作用。且随着处理时间和石墨烯浓度的增加，植物幼苗生长的所有指标，包括根/地上部鲜重和干重、根长、根尖数、株高和叶面积均相应降低。另外，黄瓜幼苗比玉米幼苗对石墨烯更加的敏感。进一步研究发现，石墨烯与黄瓜幼苗根部直接接触导致的物理损伤、氧化损伤，以及营养耗竭是其致毒机理。而石墨烯对玉米幼苗的致毒机理包括物理损伤和营养耗竭。本研究为石墨烯的环境风险评价提供了基础数据。

English Abstract

Phytotoxicity of Graphene to Higher Plants’ Seedlings and Its Mechanisms

Xu Yanan¹,
Xu Lina²

1. Ministry of Education Key Laboratory of Marine Environment and Ecology, Ocean University of China, Qingdao 266100, China;
2. College of Landscape Architecture and Forestry, Qingdao Agricultural University, Qingdao 266100, China

Received Date: 2019-07-11

Fund Project:

Keywords:

Abstract: With the extensive application and potential release of graphene materials, the negative effects of graphene have attracted great attention. To investigate the effects of graphene on the growth of higher plants, toxicity and related mechanisms of graphene on cucumber and maize seedlings were studied. After exposure to graphene (10, 50, 100, 500, 1 000 and 2 000 mg·L^-1) for 15 days under hydroponic conditions, the growth of the seedlings was inhibited. In addition, all the growth indexes, including root/shoot fresh weight and dry weight, root length, number of root tips, plant height and leaf area were also inhibited, and the negative effects were concentration and time-dependent. It was noted that cucumber seedlings were more sensitive to graphene than maize seedlings. Furthermore, physical damage, nutrient depletion, and oxidative stress were the main toxic mechanisms for graphene on cucumber seedlings. While, the toxicity mechanisms of graphene on maize seedlings were physical damage, and nutrient depletion. This study provided useful information for risk assessment of graphene in environment.