摘要:
论文研究了丙烯酰胺(acrylamide, AA)低剂量多代持续暴露对模式生物秀丽隐杆线虫(Caenorhabditis elegans, C. elegans)的发育、生殖、寿命和捕食行为能力的影响。将线虫暴露于不同剂量水平的AA中,检测对当代线虫的发育周期、后代数量、寿命和捕食行为的影响,结果显示,1.0 mmol·L-1 AA水平可诱发当代线虫发育周期延长和后代数量减少(P<0.05),0.05 mmol·L-1和0.001 mmol·L-1水平可分别诱发捕食行为能力下降和寿命缩短(P<0.05)。进一步选取对当代线虫不产生明显损伤效应的AA剂量,将线虫进行多代持续暴露处理,结果显示,0.01 mmol·L-1 AA分别可在第2代g1和第3代g2诱发发育周期延长和后代数量减少(P<0.05),0.005 mmol·L-1 AA可在第2代g1引起摄食行为能力下降,0.0001 mmol·L-1 (约7 μg·L-1,低于推测人的平均AA暴露水平)的AA在第2代g1即可诱发寿命缩短(P<0.05)。以上结果表明AA生物学毒性可以在后代间积累,极低剂量AA多代持续暴露仍可诱发生物学损伤。
Abstract:
The development, reproduction, longevity and feeding ability of the model organism Caenorhabditis elegans (C. elegans) were studied when exposed to different doses of acrylamide (AA) with multiple generations. The results showed that the development period in current generation was extended and the brood size was decreased in the next generation of C. elegans when exposed to 1.0 mmol·L-1 AA. The doses of 0.05 mmol·L-1 and 0.001 mmol·L-1 AA could notably shorten the life span and damage the feeding ability, respectively. The nematodes were further exposed to AA under the dose without significant negative effects in the current generation. A significantly extended development period in 2nd generation (g1) and decreased brood size in 3nd generation (g2) was detected in C. elegans when exposed to 0.01 mmol·L-1 AA. Feeding ability decreased in g1 generation of C. elegans when exposed to 0.005 mmol·L-1 AA, and a shortened life span was detected in g1 generation of C. elegans when exposed to 0.0001 mmol·L-1 AA (about 7 μg·L-1, which was lower than the predicted dietary level of AA in human). These results indicate that the biological toxicity of AA could be accumulated in later generations.
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