不能将值 NULL 插入列 'id',表 'aje_website.dbo.t_web_site_access';列不允许有空值。INSERT 失败。 语句已终止。 欢迎访问《生态毒理学报》编辑部网站!

首页 | 期刊简介 | 编委会 | 投稿要求 | 化学品管理信息动态 | 学术会议 | 专辑 | 毒理学测试技术设备 | 订购本刊 | 联系我们 | English version

李祥英,李志鸿,张宏涛,谭建仪,孙国萍*. 吡唑醚菌酯对不同阶段斑马鱼的毒性效应评价[J]. 生态毒理学报, 2017, 12(4): 234-241
吡唑醚菌酯对不同阶段斑马鱼的毒性效应评价
Evaluation of Toxicity Effects of Pyraclostrobin via Multiple Stage Zebrafish Assays
投稿时间:2017-05-31  修订日期:2017-08-07
DOI:10.7524/AJE.1673-5897.20170531003
中文关键词:  吡唑醚菌酯  斑马鱼  胚胎  仔鱼  毒性
英文关键词:pyraclostrobin  zebrafish  embryo  larvae  toxicity
基金项目:广东省科技计划项目(2015A030302002)
作者单位
李祥英,李志鸿,张宏涛,谭建仪,孙国萍* 佛山市环境健康与安全评价研究中心佛山 528000 
摘要点击次数: 174
全文下载次数: 28
中文摘要:
      为评价吡唑醚菌酯对鱼类不同生命阶段的毒性效应,以斑马鱼(Brachydanio rerio)为测试生物,测定了其对斑马鱼胚胎、仔鱼(孵化3 d和孵化12 d)和成鱼的急性毒性及对胚胎和仔鱼(孵化3 d)的发育毒性。结果表明,吡唑醚菌酯对斑马鱼胚胎、仔鱼(孵化3 d)、仔鱼(孵化12 d)和成鱼的96 h-LC50分别为0.0578(0.0540~0.0631)、0.0299(0.0282~0.0320)、0.0330(0.0310~0.0350)和0.0756(0.0692~0.0862) mg L-1,其毒性大小依次为仔鱼(孵化3 d)>仔鱼(孵化12 d)>胚胎>成鱼。与对照组相比,吡唑醚菌酯0.101 mg L-1处理,可对斑马鱼胚胎24 h自主运动产生显著抑制作用;0.0609、0.0777和0.101 mg L-1处理,可对斑马鱼胚胎48 h心跳速率产生显著抑制作用;0.0609和0.0777 mg L-1处理,可对斑马鱼胚胎96 h孵化仔鱼体长产生显著抑制作用。在供试浓度下吡唑醚菌酯暴露96 h后,均可对斑马鱼仔鱼(孵化3 d)体重和体长产生明显的抑制效应,对斑马鱼仔鱼(孵化3 d)心跳未产生明显影响。在高浓度下,吡唑醚菌酯可诱导斑马鱼胚胎产生畸形,主要包括心包水肿、卵黄囊水肿等;而对仔鱼(孵化3 d)未观察到明显的致畸效应。
  
AuthorAffiliation
Li Xiangying, Li Zhihong, Zhang Hongtao, Tan Jianyi, Sun Guoping*Foshan Center for Environmental Health & Safety Assessment, Fo’shan 528000, China
英文摘要:
      In order to assess the toxicity effects of pyraclostrobin to different life stages of fish, zebrafish (Brachydanio rerio) during embryo, larvae (hatched 3 days and hatched 12 days) and adult stages were selected to evaluate the acute toxicity as well as the development effects. The results showed that the 96 h-LC50 values of pyraclostrobin to embryos, larvae (hatched 3 days), larvae (hatched 12 days) and adult of zebrafish were 0.0578(0.0540-0.0631), 0.0299(0.0282-0.0320), 0.0330(0.0310-0.0350) and 0.0756(0.0692-0.0862) mg L-1, respectively. The toxicity of pyraclostrobin to four different life stages of zebrafish as follows: larvae (hatched 3 days)>larvae (hatched 12 days)>embryo>adult fish. Compared with control groups, the spontaneous movements of 24 hpf embryos was significantly decreased by pyraclostrobin at concentration of 0.101 mg L-1. The heart beat of the embryos was significantly inhibited by 0.0609, 0.0777 and 0.101 mg L-1 pyraclostrobin at 48 hpf. Furthermore, 0.0609 and 0.0777 mg L-1 pyraclostrobin could significantly reduce body length of hatched larvae at 96 hpf. At the higher test concentrations, pyraclostrobin induced a suite of morphological abnormalities during the embryonic development, including pericardial edema, yolk sac edema and melanin reduction. However, pyraclostrobin did not induce apparent abnormities in larvae (hatched 3 days).
查看全文  查看/发表评论  下载PDF阅读器
关闭

您是第3677629位访问者   京ICP备 09058833 号

主办单位:中国科学院生态环境研究中心     单位地址:北京市海淀区双清路18号

 服务热线:010-62941072       传真:010-62923563       邮编:100085    Email: stdlxb@rcees.ac.cn

    本系统由北京勤云科技发展有限公司设计

0463美女美女3764美女美女ktv