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李峰*. 体育场馆内PM2.5暴露对运动大鼠行为学及相关无氧代谢酶活性的影响[J]. 生态毒理学报, 2018, 13(2): 112-119
体育场馆内PM2.5暴露对运动大鼠行为学及相关无氧代谢酶活性的影响
The Effects of PM2.5 Exposure on the Behavior of Sports Rats and the Activity of Anaerobic Metabolic Enzymes in the Stadium
投稿时间:2017-05-10  修订日期:2017-09-27
DOI:10.7524/AJE.1673-5897.20170510001
中文关键词:  细颗粒物  行为学  代谢酶  体育馆
英文关键词:fine particulates  behavior  metabolism enzymes  gymnasium
基金项目:陕西省社会科学基金(2017R007);人才科技基金(6040300539);人文社科研究基金(6040516116)
作者单位
李峰* 西安建筑科技大学体育学院西安 710055 
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中文摘要:
      通过非暴露式气管滴注法建立损伤模型,按照低、中、高3种剂量细颗粒物(PM2.5)进行染毒,以研究不同浓度PM2.5对运动大鼠行为学及部分无氧代谢酶活性的影响。实验选取32只雄性Wistar SPF(specific pathogen free,SPF)大鼠随机分为运动对照组(EC)、高剂量运动组(30 mg kg-1) (HPE)、中剂量运动组(15 mg kg-1) (MPE)、低剂量运动组 (7.5 mg kg-1) (LPE),利用卒中指数和神经病学症状评分对大鼠的行为学进行评价,通过酶联免疫法(ELISA)测定大鼠血清、肺泡灌洗液(BALF)以及股四头肌组织中己糖激酶(HK)、丙酮酸激酶(PK)、磷酸果糖激酶(PFK-1)的活性。结果表明,与EC相比,3个剂量暴露组中,卒中指数和神经病学症状评分差异均有统计学意义;LPE、MPE、HPE组各组织中HK、PK、PFK-1活性均下降,差异有统计学意义(P<0.05 或 P<0.01)。综上所述,急性PM2.5暴露可对大鼠行为学产生不利的影响,使大鼠部分组织的无氧代谢酶酶活性降低,影响机体的运动能力。
  
AuthorAffiliation
Li Feng*College of Physical Education, Xi'an University of Architecture & Technology, Xi'an 710055, China
英文摘要:
      Discuss the mechanism of PM2.5 to sugar metabolism through studying the influence of different concentration of PM2.5 on incremental exercise rats. The 32 male Wistar SPF (specific pathogen free, SPF) rats were randomly selected and divided into control group (EC), high dose of PM2.5+exercise group (HPE), middle dose of PM2.5+exercise group (MPE) and low dose of PM2.5+ exercise group (LPE), and PM2.5 was administered to rats by intratracheal instillation at the doses of 30 mg kg-1 bw,15 mg kg-1 bw and 7.5 mg kg-1 bw respectively. Then all rats were trained by treadmill running with increasing load . Stroke index score and neurologic symptoms were used to evaluate the rats’ behavior, and the HK, PK, PFK-1 were tested by ELISA. The stroke index were statistically significant (P<0.05 or P<0.01) in three group, compared with the control group; as a result, the activity of HK, PK, PFK-1 were declined in LPE, MPE, HPE compared with the EC, and the difference was statistically significant. The PM2.5 exposure can affect rats’ behavior, resulting in changes of the anaerobic metabolizing enzymes, then reducing the body's exercise capacity. With the increase of PM2.5 concentration, HK, PK, PFK-1 activity decreased.
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