铅暴露对中华大蟾蜍耳后腺分泌蟾蜍甾烯的影响

王佳佳; 周婧; 马宏跃; 段金廒; 朱振华; 钱大玮; 吴启南

doi:10.7524/AJE.1673-5897.20160304002

江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心, 南京中医药大学药学院, 南京 210023

作者简介: 王佳佳(1992-),女,硕士研究生,研究方向为药剂学,E-mail:448091490@qq.com

基金项目:

江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心资助项目(FJGJS-2015-15，ZDXM-1-14)；江苏省科技厅产学研项目(BY2015008-01)；江苏省六大人才高峰C类(YY-015)；国家自然科学基金(No.81673563，81102762，3901894，81274199)；江苏高校优势学科建设工程资助项目(PAPD)；国家中药标准化和江苏省科技成果转化项目(ZYBZH-C-JS-30,BA2016104))

中图分类号: X171.5

The Effect of Lead on the Levels of Bufadienolides in Venom Secreted by Bufo bufo gargarizans Cantor

Jiangsu Key Laboratory for High Technology Research of TCM Formulae and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China

Fund Project:

摘要: 采用半静水式实验方法，研究Pb²⁺暴露30 d对中华大蟾蜍(Bufo bufo gargarizans Cantor)生理状态和耳后腺分泌蟾蜍甾烯水平的影响。结果表明，随Pb²⁺浓度(0.68～5.46 mg·L^-1)升高，蜕皮和自发活动减少，存活率下降。进一步采用液相-质谱检测技术发现，0.68 mg·L^-1 Pb²⁺暴露下，10种蟾蜍甾烯水平出现1.5倍以上变化。5.46 mg·L^-1 Pb²⁺暴露下，17种蟾蜍甾烯水平发生下调(>1.5倍)，其中bufarenogin水平显著下降(P<0.05)，其他物质水平与对照组无显著性差异。这说明Pb²⁺暴露对蟾蜍耳后腺中蟾蜍甾烯物质具有潜在影响。

Abstract: The effect of Pb²⁺ exposure for 30 days on the physiological status of Bufo bufo gargarizans Cantor and on the levels of bufadienolides in toad venom were studied, using the semi-static water design. With the increase of Pb²⁺ concentration from 0.68 to 5.46 mg·L^-1, the toad’s molting and spontaneous activity were remarkably inhibited, and the toad mortality increased. Furthermore, ultra performance liquid chromatography-mass spectrometry was used to monitor the relative levels of bufadienolides in toad venom. We found 10 kinds of bufadienolides with more than 1.5-fold change in 0.68 mg·L^-1 Pb²⁺ exposure group compared with control group. In Pb²⁺ exposure group (5.46 mg·L^-1), 17 kinds of bufadienolides were down-regulated with more than 1.5-fold change, in which the levels of bufarenogin was decreased significantly (P<0.05), while others had no significant change. These illustrated that Pb²⁺ exposure could affect the levels of bufadienolides in toad venom.

Key words:

铅暴露对中华大蟾蜍耳后腺分泌蟾蜍甾烯的影响

作者简介: 王佳佳(1992-),女,硕士研究生,研究方向为药剂学,E-mail:448091490@qq.com
江苏省方剂高技术研究重点实验室/江苏省中药资源产业化过程协同创新中心, 南京中医药大学药学院, 南京 210023

收稿日期: 2016-03-04

基金项目:

关键词:

The Effect of Lead on the Levels of Bufadienolides in Venom Secreted by Bufo bufo gargarizans Cantor

Received Date: 2016-03-04

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Keywords:

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