摘要:
通过气管滴注的方法,研究不同浓度细颗粒物(PM2.5)对运动大鼠行为学及不同器官的毒性作用,探讨PM2.5暴露对运动状态下机体的急性毒理作用及其可能的机制。将32只雄性Wistar SPF (Specific Pathogen Free,SPF)大鼠随机分为运动对照组(EC)、低剂量PM2.57.5 mg·(kg·bw)-1+运动组(LPE)、中剂量PM2.515 mg·(kg·bw)-1+运动组(MPE)、高剂量PM2.530 mg·(kg·bw)-1+运动组(HPE)。采用一次性气管滴注染毒后进行递增负荷跑台训练,利用卒中指数对运动过程中大鼠进行行为学评分,并测定大鼠肺、肝脏、股四头肌组织谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px),活性氧(reactive oxygen species,ROS),核转录因子核转录因子kappa B (nuclear factor-B,NF-κB)和单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)浓度。与运动对照组相比,3个暴露组中大鼠的卒中指数差异均有统计学意义(p < 0.05或p < 0.01);PM2.5暴露组GSH-Px下降,差异均有统计学意义(p< 0.05或p < 0.01);ROS,NF-κB和MCP-1上升差异均有统计学意义(p < 0.05或p < 0.01),并呈现出剂量相关性。急性PM2.5暴露可对大鼠行为学产生不利的影响,导致不同器官的抗氧化及免疫指标发生改变,影响机体的运动能力,造成某些生理损伤。
关键词:
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细颗粒物
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卒中
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抗氧化酶
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免疫
Abstract:
The 32 male Wistar SPF(Specific Pathogen Free, SPF) rats were randomly selected and divided into blank control group (QC), exercise control group (EC), low dose of PM2.5 + exercise group (LPE), middle dose of PM2.5 + exercise group(MPE), high dose of PM2.5 + exercise group (HPE). PM2.5 was administered to rats by intratracheal instillation at the doses of 7.5 mg·(kg·bw)-1, 15 mg·(kg·bw)-1 and 30 mg·(kg·bw)-1 respectively, then all rats were trained by treadmill running with increasing load. Stroke index score was used to evaluate the rats'behavior and the GSH-Px, ROS, NF-κB and MCP-1 were tested. The stroke index were statistically significant (p < 0.05 or p < 0.01) in three group compared with the control group; the activity of GSH-Px decreased after PM2.5 exposed, and the differences were statistically significant (p < 0.05 or p < 0.01). The index of ROS, NF-κB and MCP-1 was statistically increased (p < 0.05 or p < 0.01), which showed dose-related. Acute exposure by PM2.5 can be adversely affected rats' behavior, resulting in some changes of the anti-oxidant and immune. Accordingly, the body's sports ability were affected and some physiological damage occurred.