摘要:
为了探讨一次性递增负荷运动及不同浓度细颗粒物(PM2.5)暴露对大鼠己糖激酶(hexokinase,HK)-丙酮酸激酶(pyruvatekinase,PK)及异柠檬酸脱氢酶(isocitratedehydrogenase,IDH)活性影响的机制,将30只雄性Wistar SPF (SpecificPathogen Free,SPF)大鼠随机分为安静(QC)、运动对照组(EC)、低剂量PM2.5+运动组(LPE)、中剂量PM2.5+运动组(MPE)、高剂量PM2.5+运动组(HPE),采用一次性气管滴注染毒后进行递增负荷跑台训练,并通过酶联免疫法(enzyme-linked immunosorbent assay,ELISA)测定大鼠血清及肝脏组织HK,PK,IDH活性。结果表明,与安静组相比,运动对照组HK,PK酶活性下降,IDH活性升高,差异有统计学意义(p < 0.01)。和EC组相比,LPE,MPE,HPE组各组织中HK,PK,IDH活性均下降,差异有统计学意义(p < 0.05或p < 0.01)。实验表明,运动可以降低大鼠的HK,PK活性而增强IDH活性;随着浓度的增加,HK,PK,IDH活性与PM2.5暴露剂量具有相关性降低。
关键词:
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细颗粒物
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糖代谢
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限速酶
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糖酵解
Abstract:
The mechanism in the enzyme activities of hexokinase(HK), pyruvatekinase(PK) and isocitratedehydrogenase(IDH) of rats was studied after rats taking the exercise and being exposed by PM2.5. The 30 male Wistar SPF (Specific Pathogen Free, SPF) rats were randomly selected and divided into blank control group(QC), exercise control group(EC), low dose of PM2.5 + exercise group(LPE), middle dose of PM2.5 + exercise group(MPE), high dose of PM2.5 + exercise group(HPE). PM2.5 was administered to rats by intratracheal instillation at the doses of 7.5 mg ·(kg·bw)-1, 15 mg·(kg·bw)-1 and 30 mg·(kg·bw)-1 respectively, then all rats were trained by treadmill running with increasing load. The activity of HK, PK, IDH of serm and liver were tested by enzyme-linked immunosorbent assay (ELISA). The result of the experiment shows that the activity of HK and PK in EC were declined, however, the IDH was rised compared with the QC. Compared with the EC group, the activity of HK, PK, IDH in LPE, MPE, HPE were decreased with the increasing of PM2.5 concentration which is a negative relationship (p < 0.05 or p < 0.001).
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