摘要:
为认识藻红外联合作用评价法的稳定性,用苯(C6H6)、甲苯(C6H8)、二甲苯(C8H10)、氯苯(C6H5Cl)、三氯甲烷(CHCl3)和羊角月牙藻(Selenastrum capricornutum)进行了重现性实验,通过测试实验藻对5种药品不同组合的响应温差,用联合偏差系数法评价联合作用类型,分析药品联合毒性的重现性及联合作用评价法的评价效果。结果表明:二元组合中,重现性50%的5组,重现性75%的5组,平均重现性62.5%;三元组合中,重现性50%的1组,重现性75%的8组,重现性100%的1组,平均重现性75%;四元组合中,重现性50%的1组,重现性75%的1组,重现性100%的3组,平均重现性85%;五元组合重现性100%,混合药品总平均重现性73.1%。重现性大于阈值的组合19个,占全部26个组合的73.1%。药品组合元数增减与联合作用的重现性正相关。分析可知,藻红外联合作用评价法用于联合作用分析稳定性较好。
Abstract:
Evaluation method for combined toxicity effects is the core part of the measure of algae infrared radiation for acute toxicity. The stability of this method decides the use efficiency and promotional value of this testing technology. To research stability of this method, Selenastrum capricornutum and C6H6, C6H8, C8H10, C6H5Cl, CHCl3 had been used in the experiment. According to testing the difference in temperature of the algae to combinations of five kinds of chemicals, combined effects and the reproducibility of experimental results were analyzed, then evaluation effect of the method of combined deviation coefficient was judged. The results showed that in combinations of two drugs the reproducibility in five groups were 50%, which in other five groups were 75%, and the average reproducibility in ten groups was 62.5%; in ternary combinations, the reproducibility was 50% and 100% for each group, and other groups were 75%, so the average reproducibility in ten groups was 75%. The reproducibility in three groups was 100%, other groups were 75% and 100%, and the average reproducibility in five groups was 85% in quaternary combinations; the reproducibility in combinations of five drugs was 100%. The average reproducibility of all the combinations was 73.1%. The rates of reproducibility of 19 combinations were greater than that of the threshold, accounting for 73.1% of all 26 combinations. There was a positive correlation between the increase in the number of drugs and the reproducibility of combined. By means of above results, algae infrared evaluation method of combined effects had better stability for the analysis of the combined effects of toxic and hazardous substances.