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塔娜,房彦军,林本成,张伟,等. 磷酸三(2,3-二氯丙基)酯阻燃剂对稀有鮈鲫的毒性效应[J]. 生态毒理学报, 2013, 8(5): 757-762
磷酸三(2,3-二氯丙基)酯阻燃剂对稀有鮈鲫的毒性效应
Toxic Effect of Tri(2,3-Dichloroprophyl) Phosphate Flame Retardant on Rare Minnow (Gobiocypris rarus)
投稿时间:2013-01-16  修订日期:2013-04-09
DOI:10.7524/AJE.1673-5897.20130116002
中文关键词:  磷酸三(2,3-二氯丙基)酯  稀有鮈鲫  毒性效应  基因表达
英文关键词:tri(2,3-dichloroprophyl) phosphate  rare minnow  toxic effect  gene expression
基金项目:国家自然科学青年基金(20707039);天津市科技攻关课题(11ZCKFSF01100)
作者单位
塔娜 军事医学科学院卫生学环境医学研究所 天津环境与食品安全风险监控技术重点实验室天津 300050 
房彦军 军事医学科学院卫生学环境医学研究所 天津环境与食品安全风险监控技术重点实验室天津 300050 
林本成 军事医学科学院卫生学环境医学研究所 天津环境与食品安全风险监控技术重点实验室天津 300050 
张伟 军事医学科学院卫生学环境医学研究所 天津环境与食品安全风险监控技术重点实验室天津 300050 
田蕾 军事医学科学院卫生学环境医学研究所 天津环境与食品安全风险监控技术重点实验室天津 300050 
袭著革 军事医学科学院卫生学环境医学研究所 天津环境与食品安全风险监控技术重点实验室天津 300050 
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中文摘要:
      为研究新型阻燃剂磷酸三(2,3-二氯丙基)酯(TDCPP)的毒性效应,以稀有鮈鲫(Gobiocypris rarus)为实验生物,采用半静态实验方法,分别进行96 h和28 d的染毒,研究TDCPP对稀有鮈鲫的急性毒性和慢性毒性效应,并通过对稀有鮈鲫脑组织中与神经纤维的生长、发育、轴突再生等相关基因mRNA表达量的检测,初步探讨了TDCPP的神经毒性作用。结果表明:TDCPP对稀有鮈鲫的96 h-LC50为2.99(2.20~3.38) mg·L-1,根据化学物质对鱼类毒性分级标准TDCPP属于剧毒性。经0.9、1.5、2.1和2.7 mg·L-1 TDCPP染毒28 d,与对照组相比,暴露组稀有鮈鲫肝脏及脑组织中SOD和GSH-Px的活性均受到抑制,且随着TDCPP的暴露浓度增加,其抑制作用显著增强;暴露组稀有鮈鲫脑组织中与神经纤维的生长、发育相关的微管蛋白α/β、神经丝NF-M以及关键蛋白GAP-43等基因的mRNA表达量,与对照相比均发生显著下调。可知,TDCPP暴露可以诱发稀有鮈鲫神经毒性作用。
                 
AuthorAffiliation
TanaTianjin Key Laboratory of Risk Assessment & Control for Environment & Food Safety, Institute of Hygienic and Environmental Medicinal Science, Academy of Military Medical Sciences, Tianjin 300050, China
Fang YanjunTianjin Key Laboratory of Risk Assessment & Control for Environment & Food Safety, Institute of Hygienic and Environmental Medicinal Science, Academy of Military Medical Sciences, Tianjin 300050, China
Lin BenchengTianjin Key Laboratory of Risk Assessment & Control for Environment & Food Safety, Institute of Hygienic and Environmental Medicinal Science, Academy of Military Medical Sciences, Tianjin 300050, China
Zhang WeiTianjin Key Laboratory of Risk Assessment & Control for Environment & Food Safety, Institute of Hygienic and Environmental Medicinal Science, Academy of Military Medical Sciences, Tianjin 300050, China
Tian LeiTianjin Key Laboratory of Risk Assessment & Control for Environment & Food Safety, Institute of Hygienic and Environmental Medicinal Science, Academy of Military Medical Sciences, Tianjin 300050, China
Xi ZhugeTianjin Key Laboratory of Risk Assessment & Control for Environment & Food Safety, Institute of Hygienic and Environmental Medicinal Science, Academy of Military Medical Sciences, Tianjin 300050, China
英文摘要:
      Toxicological effects of tri(2,3-dichloroprophyl) phosphate (TDCPP) on rare minnow (Gobiocypris rarus) were investigated using the semi-static bioassay, including 96 h acute toxicity and 28 d chronic toxicity. We also explored some special gene expression levels such as NF-M, GAP-43, Tubulin α/β, which resulted in neurotoxicity of TDCPP. Results showed that the 96 h-LC50 value of TDCPP to rare minnow was 2.99 (2.20~3.38) mg·L-1. According to the criteria for evaluating and grading toxicity of chemicals to fishes, the toxicity of TDCPP should be the hypertoxicity level. The activities of SOD and GSH-Px in both liver and brain tissues of rare minnow were inhibited in comparison with the control, after exposure to 0.9, 1.5, 2.1 and 2.7 mg·L-1 TDCPP for 28 d. The inhibition strengthened as TDCPP concentration increased. Furthermore, the expressions of specific genes in brain tissue involved in neurotrophic factor, such as NF-M, GAP-43 and microtubulin α/β were significantly down-regulated compared with the control. It is indicated that TDCPP may induce the neurotoxic effect on rare minnow.
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