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井长勤,刘振华,李文锦,张尊,等. 离子液体氯化1-辛基-3-甲基咪唑对酵母细胞增殖生长和细胞膜通透性的影响[J]. 生态毒理学报, 2013, 8(5): 799-804
离子液体氯化1-辛基-3-甲基咪唑对酵母细胞增殖生长和细胞膜通透性的影响
Effect of 1-Octyl-3-Methylimidazolium Chloride on the Growth, Cell Proliferation and Membrane Permeability in Yeast Saccharomyces cerevisiae
投稿时间:2012-12-19  修订日期:2013-03-14
DOI:10.7524/AJE.1673-5897.20121219003
中文关键词:  离子液体  酿酒酵母  增殖生长  膜通透性
英文关键词:ionic liquid  Saccharomyces cerevisiae  proliferation and growth  membrane permeability
基金项目:河南省科技攻关重点项目(12A180022)
作者单位
井长勤 1. 河南师范大学生命科学学院新乡 453007 2. 新乡医学院生命科学技术学院新乡 453003 
刘振华 新乡医学院生命科学技术学院新乡 453003 
李文锦 新乡医学院生命科学技术学院新乡 453003 
张尊 新乡医学院生命科学技术学院新乡 453003 
王林林 新乡医学院生命科学技术学院新乡 453003 
摘要点击次数: 2781
全文下载次数: 1934
中文摘要:
      为探讨咪唑类离子液体氯化1-辛基-3-甲基咪唑([C8mim][Cl])对酵母细胞的增殖生长和细胞膜通透性的影响,以不同浓度的[C8mim][Cl]处理酵母细胞,研究离子液体对酵母细胞增殖和菌落形成的影响,通过测定酵母细胞外液蛋白质和核酸的含量,判断膜通透性的大小和[C8mim][Cl]对细胞膜性结构的损伤。结果显示,0.1 mmol·L-1
[C8mim][Cl]延长了酵母细胞到达对数生长期的时间,在6~9 h之间对酵母细胞的增殖存在明显的抑制作用。1 mmol·L-1的[C8mim][Cl]使酵母细胞增殖不能到达对数生长期,对酵母细胞的增殖一直具有较强的抑制作用。随着离子液体浓度的增加,小菌落的数量增多。当平板内[C8mim][Cl]浓度达到10 mmol·L-1时,完全抑制了菌落的形成。
[C8mim][Cl]处理酵母细胞后,细胞外液中OD280、OD260的值显著升高。研究表明,细胞膜等膜性结构通透性的增加是离子液体[C8mim][Cl]抑制酵母细胞增殖生长的原因之一。
              
AuthorAffiliation
Jing Changqin1. College of Life Science, Henan Normal University, Xinxiang 453007, China 2. College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China
Liu ZhenhuaCollege of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China
Li WenjinCollege of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China
Zhang ZunCollege of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China
Wang LinlinCollege of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, China
英文摘要:
      To study the effect of 1-octyl-3-methylimidazolium chloride on the growth, cell proliferation and membrane permeability in yeast Saccharomyces cerevisiae, yeast cells were exposed to [C8mim][Cl] with different concentrations. The proliferation of yeast cells was examined and the number and size of yeast colonies were evaluated in this study. Membrane permeability and its damage by [C8mim][Cl] were also detected by measuring OD280 and OD260 of extracellular fluid. The results showed that the treatment of yeast cells with 0.1 mmol·L-1 [C8mim][Cl] extended the time to reach the logarithmic phase time, and significantly inhibited cell proliferation between 6 h and 9 h. The yeast cell proliferation was inhibited and was unable to reach the logarithmic growth phase when treated with 1 mmol·L-1[C8mim][Cl]. With increasing the concentration of [C8mim][Cl], the number of small colonies increased. When the concentration of [C8mim][Cl] reached 10 mmol·L-1, no colony was formed in the plate medium. The values of OD280 and OD260 of extracellular fluid of yeast cells increased significantly after treated with [C8mim][Cl]. The results suggested that [C8mim][Cl] inhibited the growth and proliferation of yeast cells partially through increasing cell membrane permeability.
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