摘要:
为探讨咪唑类离子液体氯化1-辛基-3-甲基咪唑([C8mim][Cl])对酵母细胞的增殖生长和细胞膜通透性的影响,以不同浓度的[C8mim][Cl]处理酵母细胞,研究离子液体对酵母细胞增殖和菌落形成的影响,通过测定酵母细胞外液蛋白质和核酸的含量,判断膜通透性的大小和[C8mim][Cl]对细胞膜性结构的损伤。结果显示,0.1 mmol·L-1的[C8mim][Cl]延长了酵母细胞到达对数生长期的时间,在6~9 h之间对酵母细胞的增殖存在明显的抑制作用。1 mmol·L-1的[C8mim][Cl]使酵母细胞增殖不能到达对数生长期,对酵母细胞的增殖一直具有较强的抑制作用。随着离子液体浓度的增加,小菌落的数量增多。当平板内[C8 mim][Cl]浓度达到10 mmol·L-1时,完全抑制了菌落的形成。[C8 mim][Cl]处理酵母细胞后,细胞外液中OD280、OD260的值显著升高。研究表明,细胞膜等膜性结构通透性的增加是离子液体[C8mim][Cl]抑制酵母细胞增殖生长的原因之一。
Abstract:
To study the effect of 1-octyl-3-methylimidazolium chloride on the growth, cell proliferation and membrane permeability in yeast Saccharomyces cerevisiae, yeast cells were exposed to[C8 mim] [Cl] with different concentrations. The proliferation of yeast cells was examined and the number and size of yeast colonies were evaluated in this study. Membrane permeability and its damage by[C8mim] [Cl] were also detected by measuring OD280 and OD260 of extracellular fluid. The results showed that the treatment of yeast cells with 0.1 mmol·L-1[C8mim] [Cl] extended the time to reach the logarithmic phase time, and significantly inhibited cell proliferation between 6 h and 9 h. The yeast cell proliferation was inhibited and was unable to reach the logarithmic growth phase when treated with 1 mmol·L-1[C8 mim] [Cl]. With increasing the concentration of[C8 mim] [Cl], the number of small colonies increased. When the concentration of[C8mim] [Cl] reached 10 mmol·L-1, no colony was formed in the plate medium. The values of OD280 and OD 260 of extracellular fluid of yeast cells increased significantly after treated with[C8mim] [Cl]. The results suggested that[C8mim] [Cl] inhibited the growth and proliferation of yeast cells partially through increasing cell membrane permeability.
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