摘要:
为了探讨苯和甲醛联合染毒对小鼠脾脏的损伤以及二者是否具有一定的协同作用,选择BALB/c雄性小鼠30只,随机分为5组,每组6只,设苯组、甲醛组、苯和甲醛联合染毒组及玉米油对照组和空白对照组,对小鼠进行气态甲醛吸入染毒或/和苯玉米油溶液灌胃染毒。染毒结束后对小鼠脾脏进行组织病理学观察,并且测定脾脏的脏器系数以及脾脏组织的活性氧 (ROS)和丙二醛(MDA)的含量。结果显示,苯和甲醛联合染毒组小鼠脾脏组织中ROS的含量与空白对照组相比有极显著的上升趋势(P<0.01),脾脏的脏器系数以及脾脏组织中MDA的含量与空白对照组相比有显著性差异(P<0.05)。此外,在脾脏免疫组织形态变化以及脾脏组织中MDA含量这2个生理生化指标上,苯和甲醛联合染毒对小鼠脾脏的损伤具有一定的协同作用。
Abstract:
To study the damage of mice spleen induced by combined exposure of benzene and formaldehyde, and whether it has certain synergetic effect, 30BALB/c male mice were chosen and then divided randomly into different groups:benzene group, formaldehyde group, combined exposure group of benzene and formaldehyde, corn oil control group and untreated control group. After treatment, the mice were killed, the contents of ROS and MDA in the mice spleen were measured, the organ coefficient of mice spleen was counted and the sections of mice spleen were made. The results showed that the contents of ROS in the spleen of combined exposure group have very significant increase (P<0.01) compared with untreated control group, and the organ coefficient of spleen and the content of MDA in the spleen have significant difference (P<0.05). In addition, in terms of these biomarkers that are the content of MDA in the spleen and the change of spleen immune morphology, we can infer that the damage of mice spleen induced by benzene combing with formaldehyde has certain synergenic effect.
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