摘要:
以斑马鱼(Danio rerio)为研究对象,探讨铅(Pb)、得克隆(DP)及二者联合急性暴露对斑马鱼胚胎的神经毒性作用。结果表明,Pb(5、20 μg·L-1)和DP(15、60 μg·L-1)单独暴露均会引起斑马鱼自主运动频率增加,触摸反应能力和自由游泳活力下降,并且抑制初级运动神经元的生长,加剧尾部细胞凋亡。但与20 μg·L-1 Pb单独暴露相比,高剂量联合暴露(20 μg·L-1 Pb + 60 μg·L-1 DP)使斑马鱼的自主运动频率显著降低(P < 0.05),触摸反应能力和自由游泳活力显著增强(P < 0.05),初级运动神经元轴突长度显著增加(P < 0.05),尾部细胞凋亡减少。与5 μg·L-1 Pb单独暴露相比,低剂量联合暴露(5 μg·L-1 Pb + 15 μg·L-1 DP)也显著减少斑马鱼尾部的细胞凋亡(P < 0.05)。上述结果表明,Pb或DP单独暴露对斑马鱼均可引起神经毒性作用;但二者联合暴露对斑马鱼自主运动、触摸反应以及自由游泳活力的影响则表现为拮抗作用。
关键词:
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铅
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得克隆
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斑马鱼
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胚胎
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神经毒性
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联合暴露
Abstract:
Neurotoxic effects of acute exposure to lead (Pb) or Dechlorane Plus (DP), or both were investigated using zebrafish (Danio rerio) embryos. Results showed that exposure to Pb (5, 20 μg·L-1) or DP (15, 60 μg·L-1) alone increased spontaneous movement, decreased touch response and free-swimming activity, inhibited axonal growth of primary motoneuron and induced cell apoptosis in zebrafish embryos. Co-exposure to 20 μg·L-1 Pb and 60 μg·L-1 DP significantly decreased spontaneous movement (P < 0.05), enhanced touch response and free-swimming activity (P < 0.05), increased axonal length of primary motoneuron (P < 0.05) and reduced cell apoptosis in zebrafish when compared to 20 μg·L-1 Pb exposure alone. Co-exposure to 5 μg·L-1 Pb and 15 μg·L-1 DP also significantly decreased cell apoptosis on the tail region when compared to 5 μg·L-1 Pb exposure alone (P < 0.05). These results demonstrated that Pb or DP exposure alone could induce neurobehavioral toxicity in zebrafish, but Pb and DP co-exposure had antagonistic effects on spontaneous movements, touch response and free swimming activity.