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荧光染色法测定活性污泥中的活性生物量

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龙海涛, 齐鲁, 刘国华, 庞毓旻, 庄健, 李小冬, 王洪臣. 荧光染色法测定活性污泥中的活性生物量[J]. 环境工程学报, 2015, 9(2): 683-686. doi: 10.12030/j.cjee.20150229
引用本文: 龙海涛, 齐鲁, 刘国华, 庞毓旻, 庄健, 李小冬, 王洪臣. 荧光染色法测定活性污泥中的活性生物量[J]. 环境工程学报, 2015, 9(2): 683-686. doi: 10.12030/j.cjee.20150229
shu
Long Haitao, Qi Lu, Liu Guohua, Pang Yumin, Zhuang Jian, Li Xiaodong, Wang Hongchen. Measurement of active biomass in activated sludge by fluorescence assay[J]. Chinese Journal of Environmental Engineering, 2015, 9(2): 683-686. doi: 10.12030/j.cjee.20150229
Citation: Long Haitao, Qi Lu, Liu Guohua, Pang Yumin, Zhuang Jian, Li Xiaodong, Wang Hongchen. Measurement of active biomass in activated sludge by fluorescence assay[J]. Chinese Journal of Environmental Engineering, 2015, 9(2): 683-686. doi: 10.12030/j.cjee.20150229
shu

荧光染色法测定活性污泥中的活性生物量

  • 1.

    中国人民大学环境学院, 北京 100872

  • 基金项目:

    国家"863"高技术研究发展计划项目(2009AA063804)

    国家"水体污染控制与治理"科技重大专项(2011ZX07316-001)

  • 中图分类号: X172

Measurement of active biomass in activated sludge by fluorescence assay

  • 1.

    School of Environment & Natural Resources, Renmin University of China, Beijing 100872, China

  • Fund Project:

  • 摘要: 活性污泥中活性微生物量是表征污染物去除能力的重要指标。探索了荧光染色法直接检测活性细菌的原理和测定方法,结果表明,大肠杆菌、唾液链球菌和铜绿假单胞杆菌的活菌细胞浓度与荧光光强的线性相关度都超过0.95;在惰性颗粒物浓度为20 mg/L到60 mg/L的范围内,其对荧光光强的影响可通过线性关系修正;搅拌速度为1 000 r/min且搅拌10 min时前处理效果最好。
    Abstract: Active biomass was an important index of pollutants removal ability. Principle and assay method of fluorescence assay on active bacteria was studied. The results showed that the linearly dependent coefficients between bacterial including Escherichia coli, Streptococcus saliarius and Pseudomonas aeruginosa and fluorescence intensities all exceeded 0.95. When the concentration of the inert particulate matter was ranged from 20 mg/L to 60 mg/L, the impact of inert particulate matter on fluorescence assay can be linearity corrected. The effect of pretreatment was best when agitation rate was 1 000 r/min and agitation time was 10 minutes.
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  • 收稿日期:  2014-02-17
  • 刊出日期:  2015-02-07
龙海涛, 齐鲁, 刘国华, 庞毓旻, 庄健, 李小冬, 王洪臣. 荧光染色法测定活性污泥中的活性生物量[J]. 环境工程学报, 2015, 9(2): 683-686. doi: 10.12030/j.cjee.20150229
引用本文: 龙海涛, 齐鲁, 刘国华, 庞毓旻, 庄健, 李小冬, 王洪臣. 荧光染色法测定活性污泥中的活性生物量[J]. 环境工程学报, 2015, 9(2): 683-686. doi: 10.12030/j.cjee.20150229
shu
Long Haitao, Qi Lu, Liu Guohua, Pang Yumin, Zhuang Jian, Li Xiaodong, Wang Hongchen. Measurement of active biomass in activated sludge by fluorescence assay[J]. Chinese Journal of Environmental Engineering, 2015, 9(2): 683-686. doi: 10.12030/j.cjee.20150229
Citation: Long Haitao, Qi Lu, Liu Guohua, Pang Yumin, Zhuang Jian, Li Xiaodong, Wang Hongchen. Measurement of active biomass in activated sludge by fluorescence assay[J]. Chinese Journal of Environmental Engineering, 2015, 9(2): 683-686. doi: 10.12030/j.cjee.20150229
shu

荧光染色法测定活性污泥中的活性生物量

  • 1. 中国人民大学环境学院, 北京 100872
  • 收稿日期:  2014-02-17
基金项目:

国家"863"高技术研究发展计划项目(2009AA063804)

国家"水体污染控制与治理"科技重大专项(2011ZX07316-001)

摘要: 活性污泥中活性微生物量是表征污染物去除能力的重要指标。探索了荧光染色法直接检测活性细菌的原理和测定方法,结果表明,大肠杆菌、唾液链球菌和铜绿假单胞杆菌的活菌细胞浓度与荧光光强的线性相关度都超过0.95;在惰性颗粒物浓度为20 mg/L到60 mg/L的范围内,其对荧光光强的影响可通过线性关系修正;搅拌速度为1 000 r/min且搅拌10 min时前处理效果最好。

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