微塑料对双齿围沙蚕生理代谢的影响研究
Effects of Microplastics on Physiological Metabolism of Perinereis aibuhitensis
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摘要: 为探讨微塑料对双齿围沙蚕生理代谢的影响，本文研究了聚丙烯（PP）、聚酰胺（PA）和聚氯乙烯（PVC）3种微塑料在不同浓度（0.1%、1%和5%）和暴露时间下对双齿围沙蚕体内过氧化氢酶（CAT）、乙酰胆碱酯酶（AchE）活性以及代谢指标排氨率和呼吸速率的影响。结果表明，随微塑料浓度的升高，双齿围沙蚕体内CAT活性显著提升，5%浓度下双齿围沙蚕体内CAT活性最高；PVC对双齿围沙蚕体内AchE活性表现为低浓度（0.1%）抑制、高浓度（5%）促进的作用；PP和PA在不同浓度下对双齿围沙蚕体内AchE酶活性均表现为促进作用；随着微塑料浓度的升高，双齿围沙蚕的排氨率和呼吸速率显著升高（P<0.05）。综上，微塑料在高浓度条件下，在一定时间内可以对双齿围沙蚕产生氧化胁迫作用，并一定程度上影响其排氨率和呼吸速率。Abstract: In order to investigate the effect of microplastics on the physiological metabolism of the bidentate silkworm, this paper studied the effect of three microplastics of polypropylene (PP), polyamide (PA) and polyvinyl chloride (PVC) at different concentrations (0.1%, 1%, 5%) and exposure time on the activities of catalase (CAT) and acetylcholinesterase (AchE), as well as the metabolic index ammonia excretion rate and respiration rate. The results show that the CAT activity of P. aibuhitensis increased significantly with the increase of microplastics concentration. The CAT activity of P. aibuhitensis is the highest under 5% microplastic concentration. The effect of PVC on the AchE activity of P. aibuhitensis is inhibited by low concentration (0.1%) and promoted by high concentration (5%). Different concentrations of PP and PA can promote the AchE activity of P. aibuhitensis. With the increase of microplastics concentration, the ammonia excretion rate and respiration rate of P. aibuhitensis increased significantly (P<0.05). In summary, the microplastics can produce oxidative stress on P. aibuhitensis within a certain period of time under high concentration, and affect its ammonia excretion rate and respiration rate to a certain extent.
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Key words:
- microplastics /
- Perinereis aibuhitensis /
- anti-oxidation /
- metabolism /
- catalase /
- acetylcholinesterase /
- ammonia excretion rate /
- respiration rate
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Wagner M, Scherer C, Alvarez-Muñoz D, et al. Microplastics in freshwater ecosystems:What we know and what we need to know[J]. Environmental Sciences Europe, 2014, 26(1):12 王西西, 曲长凤, 王文宇, 等. 中国海洋微塑料污染的研究现状与展望[J]. 海洋科学, 2018, 42(3):131-141 Wang X X, Qu C F, Wang W Y, et al. Research status and prospects of marine microplastics contamination in the Chinese oceans[J]. Marine Sciences, 2018, 42(3):131-141(in Chinese)
毕治功, 张谦, 陈云峰, 等. 聚氯乙烯应用及市场分析[J]. 弹性体, 2018, 28(5):78-82 Bi Z G, Zhang Q, Chen Y F, et al. Application and market analysis of PVC[J]. China Elastomerics, 2018, 28(5):78-82(in Chinese)
白颐. 国内外聚酰胺系列产品发展分析[J]. 化学工业, 2008, 26(10):3-8 Bai Y. Analysis on the development of polyamide products at home and abroad[J]. Chemical Industry, 2008, 26(10):3-8(in Chinese)
王红专, 高先明. 国内外聚酰胺现状与展望[J]. 化工生产与技术, 2008, 15(1):38-40 , 58, 68 Wang H Z, Gao X M. Present status and development prospect of polyamide at home and abroad[J]. Chemical Production and Technology, 2008, 15(1):38-40, 58, 68(in Chinese)
吴汾, 唐伟家. 聚酰胺工程塑料生产、市场及其趋势[J]. 上海塑料, 2009(4):17-22 Wu F, Tang W J. Production, market and its future tendency for polyamide engineering plastics[J]. Shanghai Plastics, 2009 (4):17-22(in Chinese)
Kukulka T, Proskurowski G, Morét-Ferguson S, et al. The effect of wind mixing on the vertical distribution of buoyant plastic debris[J]. Geophysical Research Letters, 2012, 39(7):7601 Lattin G L, Moore C J, Zellers A F, et al. A comparison of neustonic plastic and zooplankton at different depths near the southern California shore[J]. Marine Pollution Bulletin, 2004, 49(4):291-294 Andrady A L. Microplastics in the marine environment[J]. Marine Pollution Bulletin, 2011, 62(8):1596-1605 Thompson R C, Swan S H, Moore C J, et al. Our plastic age[J]. Philosophical Transactions of the Royal Society of London Series B, Biological Sciences, 2009, 364(1526):1973-1976 Sussarellu R, Suquet M, Thomas Y, et al. Oyster reproduction is affected by exposure to polystyrene microplastics[J]. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(9):2430-2435 赵淑江, 王海雁, 刘健. 微塑料污染对海洋环境的影响[J]. 海洋科学, 2009, 33(3):84-86 Zhao S J, Wang H Y, Liu J. Influence of microplastics pollution on marine environment[J]. Marine Sciences, 2009, 33(3):84-86(in Chinese)
时冬晴, 叶建生. 沙蚕的养殖方式及其应用开发现状[J]. 河北渔业, 2006(10):44-45, 58 Yuan X T, Chen A H, Zhou Y B, et al. The influence of cadmium on the antioxidant enzyme activities in polychaete Perinereis aibuhitensis Grube (Annelida:Polychaeta)[J]. Chinese Journal of Oceanology and Limnology, 2010, 28(4):849-855 刘慧玲, 郭文浚, 谭振华, 等. pH、亚硝酸盐和氨氮对双齿围沙蚕耗氧率和排氨率的影响[J]. 广东海洋大学学报, 2016, 36(3):52-56 Liu H L, Guo W J, Tan Z H, et al. Effects of pH, ammonia and nitrite on oxygen consumption and ammonia excretion of Perinereis aibuhitensis[J]. Journal of Guangdong Ocean University, 2016, 36(3):52-56(in Chinese)
Scaps P, Demuynck S, Descamps M, et al. Effects of organophosphate and carbamate pesticides on acetylcholinesterase and choline acetyltransferase activities of the polychaete Nereis diversicolor[J]. Archives of Environmental Contamination and Toxicology, 1997, 33(2):203-208 Durou C, Smith B D, Roméo M, et al. From biomarkers to population responses in Nereis diversicolor:Assessment of stress in estuarine ecosystems[J]. Ecotoxicology and Environmental Safety, 2007, 66(3):402-411 孙福红, 周启星, 张倩如. 石油烃、Cu2+对沙蚕的毒性效应及对其抗氧化酶系统的影响[J]. 环境科学, 2006, 27(7):1415-1419 Sun F H, Zhou Q X, Zhang Q R. Toxic effects of petroleum hydrocarbons and copper on polychaete Nereis diversicolor and on its antioxidant enzyme systems[J]. Environmental Science, 2006, 27(7):1415-1419(in Chinese)
王玲, 陈爱华, 赵啸, 等. 温度和对双齿围沙蚕呼吸和排泄的影响[J]. 大连水产学院学报, 2004, 19(3):176-181 Wang L, Chen A H, Zhao X, et al. Effect of temperature and body weight on respiration and excretion in Perinereis aibuhitensis Grube[J]. Journal of Dalian Fisheries University, 2004, 19(3):176-181(in Chinese)
刘勇, 线薇薇. 温度对日本刺沙蚕氮生长和氮收支的影响[J]. 水产科学, 2010, 29(6):311-316 Liu Y, Xian W W. The effects of temperature on nitrogen retention and nitrogen budget in Neanthes japonica[J]. Fisheries Science, 2010, 29(6):311-316(in Chinese)
Solé M, Kopecka-Pilarczyk J, Blasco J. Pollution biomarkers in two estuarine invertebrates, Nereis diversicolor and Scrobicularia plana, from a Marsh ecosystem in SW Spain[J]. Environment International, 2009, 35(3):523-531 王晶, 周启星, 张倩茹, 等. 沙蚕暴露于石油烃、Cu2+和Cd2+毒性效应及乙酰胆碱酯酶活性的响应[J]. 环境科学, 2007, 28(8):1796-1801 Wang J, Zhou Q X, Zhang Q R, et al. Toxic effects of petroleum hydrocarbons, copper and cadmium on polychaete Perinereis aibuhitensis Grube and on its responses in acetycholinesterase activity[J]. Environmental Science, 2007, 28(8):1796-1801(in Chinese)
Pérez E, Blasco J, Solé M. Biomarker responses to pollution in two invertebrate species:Scrobicularia plana and Nereis diversicolor from the Cádiz Bay (SW Spain)[J]. Marine Environmental Research, 2004, 58(2-5):275-279 Zhu L, Bai H Y, Chen B J, et al. Microplastic pollution in North Yellow Sea, China:Observations on occurrence, distribution and identification[J]. Science of the Total Environment, 2018, 636:20-29 Peng G Y, Xu P, Zhu B S, et al. Microplastics in freshwater river sediments in Shanghai, China:A case study of risk assessment in mega-cities[J]. Environmental Pollution, 2018, 234:448-456 Zhao S Y, Zhu L X, Li D J. Microplastic in three urban estuaries, China[J]. Environmental Pollution, 2015, 206:597-604 丛艺, 周建行, 孙粒钧, 等. 荧光聚苯乙烯微粒在沙蚕体内的摄入、排出及其毒性效应[J]. 海洋环境科学, 2019, 38(2):161-166 Cong Y, Zhou J H, Sun L J, et al. Ingestion, egestion and toxic effects of fluorescent polystyrene microspheres on the polychaete, Perinereis aibuhitensis[J]. Marine Environmental Science, 2019, 38(2):161-166(in Chinese)
Green D S, Boots B, Sigwart J, et al. Effects of conventional and biodegradable microplastics on a marine ecosystem engineer (Arenicola marina) and sediment nutrient cycling[J]. Environmental Pollution, 2016, 208(Pt B):426-434 周银环, 曹伏君, 罗杰, 等. 不同温度梯度和规格体重对大弹涂鱼(Boleophthalmus pectinirostris)耗氧率(RO)和排氨率(RN)的影响[J]. 海洋与湖沼, 2017, 48(2):392-397 Zhou Y H, Cao F J, Luo J, et al. Influence of temperature and body weight on oxygen consumption and ammonia excretion of Boleophthalmus pectinirostris[J]. Oceanologia et Limnologia Sinica, 2017, 48(2):392-397(in Chinese)
黄晓. 靛酚蓝法测定海水、河口水中铵氮的"盐效应"及其消除[J]. 海峡科学, 2013(7):3-6 Casas S M, Lavaud R, la Peyre M K, et al. Quantifying salinity and season effects on eastern oyster clearance and oxygen consumption rates[J]. Marine Biology, 2018, 165(5):1-13 吕林兰, 杨家新, 董学兴, 等. 马拉硫磷对双齿围沙蚕乙酰胆碱酯酶和过氧化氢酶的影响[J]. 农业环境科学学报, 2010, 29(3):431-436 Lv L L, Yang J X, Dong X X, et al. Effection of malathion on the activity of acetycholinesterase and catalase of polychaete Perinereis aibuhitensis[J]. Journal of Agro-Environment Science, 2010, 29(3):431-436(in Chinese)
王丽丽, 王轶男, 宋莹莹, 等. 镉、苯并(a)芘胁迫对双齿围沙蚕SOD、CAT活性及MDA含量的影响[J]. 海洋环境科学, 2015, 34(1):17-22 Wang L L, Wang Y N, Song Y Y, et al. Influence of single and combined cadmium and benzo(a)-pyrene on SOD, CAT activities and MDA content in the polychaete Perinereis aibuhitensis[J]. Marine Environmental Science, 2015, 34(1):17-22(in Chinese)
von Moos N, Burkhardt-Holm P, Köhler A. Uptake and effects of microplastics on cells and tissue of the blue mussel Mytilus edulis L. after an experimental exposure[J]. Environmental Science & Technology, 2012, 46(20):11327-11335 Rochman C M, Hoh E, Kurobe T, et al. Ingested plastic transfers hazardous chemicals to fish and induces hepatic stress[J]. Scientific Reports, 2013, 3:3263 de la Torre F R, Ferrari L, Salibián A. Freshwater pollution biomarker:Response of brain acetylcholinesterase activity in two fish species[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology, 2002, 131(3):271-280 Oliveira M, Ribeiro A, Hylland K, et al. Single and combined effects of microplastics and pyrene on juveniles (0+ Group) of the common goby Pomatoschistus microps (Teleostei, Gobiidae)[J]. Ecological Indicators, 2013, 34:641-647 Lee K W, Shim W J, Kwon O Y, et al. Size-dependent effects of micro polystyrene particles in the marine copepod Tigriopus japonicas[J]. Environmental Science & Technology, 2013, 47(19):11278-11283 Hämer J, Gutow L, Köhler A, et al. Fate of microplastics in the marine isopod Idotea emarginata[J]. Environmental Science & Technology, 2014, 48(22):13451-13458 Lei L L, Wu S Y, Lu S B, et al. Microplastic particles cause intestinal damage and other adverse effects in zebrafish Danio rerio and nematode Caenorhabditis elegans[J]. Science of the Total Environment, 2018, 619-620:1-8 Holloway G J, Sibly R M, Povey S R. Evolution in toxin-stressed environments[J]. Functional Ecology, 1990, 4(3):289 Mouneyrac C, Mastain O, Amiard J C, et al. Trace-metal detoxification and tolerance of the estuarine worm Hediste diversicolor chronically exposed in their environment[J]. Marine Biology, 2003, 143(4):731-744 Anderson J C, Park B J, Palace V P. Microplastics in aquatic environments:Implications for Canadian ecosystems[J]. Environmental Pollution, 2016, 218:269-280 -
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