摘要:
为研究全氟辛基磺酸(PFOS)及其替代品的内分泌干扰效应,将重组人雌激素/雄激素/孕激素/甲状腺激素/维甲酸受体基因的双杂交酵母暴露于不同浓度的PFOS及替代品中,并结合体外代谢活化技术检测直接和间接的类/抗激素活性。结果表明,被替代品PFOS表现为雄激素受体、甲状腺受体和维甲酸受体拮抗剂,且在代谢活化后新增了雌激素受体拮抗效应,其半数效应浓度(EC50)在3.0×10-4~4.8×10-3 g· L-1之间;同时,4种替代化学品没有任何效应检出,说明不会通过与核受体结合干扰天然激素的正常功能。研究结果说明,与原化学品相比,替代产品的应用在内分泌干扰效应方面可能具有更低的环境风险。
Abstract:
To study the endocrine disrupting effects of perfluorooctane sulfonate (PFOS) and its substitutes, the twohybrid recombinant yeast cells were exposed to different concentration levels of test substances to detect their hormonal activity with or without the metabolic activation technology. Results of the bioassay indicated that PFOS could act as the androgen receptor, thyroid receptor and retinoic X receptor antagonist, and additionally showed estrogen receptor antagonistic activity after the in vitro metabolic activation, with the half effective concentrations (EC50) ranging from 3.0×10-4 to 4.8×10-3 g·L-1. Simultaneously, four substitutes did not show significant effect, suggesting they did not disrupt the normal function of natural hormones by binding to the nuclear receptors. Thus, compared with PFOS, the application of substitutes may be deemed a lower environmental risk in the aspect of hormonal activity.
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