摘要:
针对国内外有关荧蒽(FLT)对种子萌发影响研究极少的现状,本文设置5个质量浓度(2,5,15,25,50 mg·L-1)的染毒组、1个空白对照组(0 mg·L-1)和1个助溶剂对照组(丙酮体积分数为1%),以大豆种子发芽率、发芽势、发芽指数、活力指数和幼苗长度为测量指标,探索性地研究了FLT对大豆种子萌发的毒性效应。结果表明:在浓度小于15 mg·L-1的FLT胁迫下,大豆发芽率与对照相比无显著差异,大于等于15 mg·L-1的FLT胁迫则显著抑制了发芽率(p < 0.05)。发芽势、发芽指数、活力指数则对FLT的胁迫作用更加敏感,当FLT浓度大于等于5 mg·L-1时呈显著降低(p < 0.05)。FLT对幼苗长度起抑制作用,且随浓度越高和处理时间增长,抑制作用逐渐增强。
Abstract:
To study the toxic effects of fluoranthene (FLT) on soybean (Glycine max) at its early growth stage, germination rate, germination vigor, germination index, germination vitality and seedling length of soybean were investigated. The results showed that germination rate did not change significantly when concentrations of FLT were lower than 15 mg·L-1, whereas it was inhibited significantly when FLT was at high concentrations (≥ 15 mg·L-1). Germination vigor, germination index and germination vitality, which decreased significantly when the concentration of FLT was higher than 5 mg L-1, were more sensitive to FLT stress than germination rate. The inhibition effect of FLT on seedling length was enhanced as the concentration and treating time increased.