摘要:
为了验证我国本土常见的稀脉浮萍(Lemna aequinoctialis)是否适用于以青萍(Lemna minor)作为标准试验生物的生长抑制试验,对两种浮萍的毒性效应终点和毒性响应差异进行比较。通过对两种浮萍的总叶面积、干重、鲜重同叶状体数之间的相关性进行分析,根据《OECD化学品测试准则No.221浮萍生长抑制试验》,遵从良好实验室规范(GLP),将两种浮萍暴露于参比物质3,5-二氯苯酚,进行7 d更新式毒性试验,暴露浓度为(1.0~10) mg·L-1。结果表明,青萍的培养条件、试验方法以及毒性效应终点均适用于稀脉浮萍,两种浮萍的毒性试验呈现出相似的时间-效应关系和剂量-效应关系,两者的毒性效应浓度具有可比性,且数值上接近。以上实验结果可以得出,稀脉浮萍适用于生长抑制试验;鉴于稀脉浮萍在我国分布广泛、容易获取,稀脉浮萍可作为毒性测试的国家标准试验生物。
Abstract:
To validate whether native Lemna aequinoctialis (commonly found in China) is suitable for the growth inhibition test using Lemna minor as standard test organism, these two Lemna species were compared regarding to the measured toxicity endpoints and the difference in responses to chemical exposure. The correlations of total frond area, fresh weight and dry weight with frond number of L. minor and L. aequinoctialis were investigated. In compliant with Good Laboratory Practices (GLP), a seven-day semi-static toxicity test was performed using the two duckweeds exposed to 3,5-dichlorophenol (as reference substance) at concentrations from 1.0 mg L-1to 10 mg L-1, according to "OECD Guidelines for the Testing of Chemicals No. 221 Lemna sp. Growth Inhibition Test". It was found that cultivation conditions, test procedures and toxicity endpoints for L. minor were applicable to L. aequinoctialis. The test of the two duckweeds showed the similar time-effect relationship as well as the dose-effect relationship; and those effect concentrations at each level were comparable and approximate. In conclusion, L. aequinoctialis can serve as test organisms as L. minor. L. aequinoctialis can become a candidate of the national standard species in toxicity testing.
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